Binding of Transcription Factors and Creation of a Large Nucleoprotein Complex on the Human Cytomegalovirus Enhancer

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Jun 1

PMID 3035545

Citations 62

Authors

P Ghazal

H Lubon

B Fleckenstein

L Hennighausen

Affiliations

Soon will be listed here.

Abstract

The effect of the human cytomegalovirus immediate early region 1 enhancer on transcription was studied in vitro with HeLa cell nuclear extract. Stimulation of in vitro transcription mediated by the enhancer element involves its recognition by specific trans-acting factors present in the nuclear extract. DNase I protection analysis was used to determine at the nucleotide level those enhancer sequences that interact with nuclear factors. At least nine sites of protein-DNA interaction were detected over approximately 400 base pairs of enhancer sequence. The regions of nuclease protection are associated with 21-, 19-, 18-, and 17-base-pair repeat elements as well as with a unique sequence, creating a large nucleoprotein complex. The relationship between the protein binding and the activity of the immediate early region 1 enhancer is discussed.

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References

WELLER T . The cytomegaloviruses: ubiquitous agents with protean clinical manifestations. I. N Engl J Med. 1971; 285(4):203-14. DOI: 10.1056/NEJM197107222850406. View

Gronostajski R . Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides. Nucleic Acids Res. 1986; 14(22):9117-32. PMC: 311933. DOI: 10.1093/nar/14.22.9117. View

Tanaka S, Otsuka M, Ihara S, Maeda F, Watanabe Y . Induction of pre-early nuclear antigen(s) in HEL cells infected with human cytomegalovirus. Microbiol Immunol. 1979; 23(4):263-71. DOI: 10.1111/j.1348-0421.1979.tb00462.x. View

DeMarchi J, Schmidt C, Kaplan A . Patterns of transcription of human cytomegalovirus in permissively infected cells. J Virol. 1980; 35(2):277-86. PMC: 288811. DOI: 10.1128/JVI.35.2.277-286.1980. View

Gibson W . Immediate-early proteins of human cytomegalovirus strains AD 169, Davis, and Towne differ in electrophoretic mobility. Virology. 1981; 112(1):350-4. DOI: 10.1016/0042-6822(81)90641-3. View

Wathen M, Stinski M . Temporal patterns of human cytomegalovirus transcription: mapping the viral RNAs synthesized at immediate early, early, and late times after infection. J Virol. 1982; 41(2):462-77. PMC: 256775. DOI: 10.1128/JVI.41.2.462-477.1982. View

Weiher H, Konig M, Gruss P . Multiple point mutations affecting the simian virus 40 enhancer. Science. 1983; 219(4585):626-31. DOI: 10.1126/science.6297005. View

Stinski M, Thomsen D, Stenberg R, Goldstein L . Organization and expression of the immediate early genes of human cytomegalovirus. J Virol. 1983; 46(1):1-14. PMC: 255088. DOI: 10.1128/JVI.46.1.1-14.1983. View

Dignam J, Lebovitz R, Roeder R . Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983; 11(5):1475-89. PMC: 325809. DOI: 10.1093/nar/11.5.1475. View

10.

McDonough S, Spector D . Transcription in human fibroblasts permissively infected by human cytomegalovirus strain AD169. Virology. 1983; 125(1):31-46. DOI: 10.1016/0042-6822(83)90061-2. View

11.

Dynan W, Tjian R . Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II. Cell. 1983; 32(3):669-80. DOI: 10.1016/0092-8674(83)90053-3. View

12.

Scholer H, Gruss P . Specific interaction between enhancer-containing molecules and cellular components. Cell. 1984; 36(2):403-11. DOI: 10.1016/0092-8674(84)90233-2. View

13.

Jahn G, Knust E, Schmolla H, Sarre T, Nelson J, McDougall J . Predominant immediate-early transcripts of human cytomegalovirus AD 169. J Virol. 1984; 49(2):363-70. PMC: 255474. DOI: 10.1128/JVI.49.2.363-370.1984. View

14.

Emerson B, Felsenfeld G . Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene. Proc Natl Acad Sci U S A. 1984; 81(1):95-9. PMC: 344617. DOI: 10.1073/pnas.81.1.95. View

15.

Elgin S . Anatomy of hypersensitive sites. Nature. 1984; 309(5965):213-4. DOI: 10.1038/309213a0. View

16.

Wu C . Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin. Nature. 1984; 311(5981):81-4. DOI: 10.1038/311081a0. View

17.

Sassone-Corsi P, Wildeman A, Chambon P . A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro. Nature. 1985; 313(6002):458-63. DOI: 10.1038/313458a0. View

18.

Wildeman A, Sassone-Corsi P, Grundstrom T, Zenke M, Chambon P . Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor. EMBO J. 1984; 3(13):3129-33. PMC: 557828. DOI: 10.1002/j.1460-2075.1984.tb02269.x. View

19.

Hennighausen L, Siebenlist U, DANNER D, Leder P, Rawlins D, Rosenfeld P . High-affinity binding site for a specific nuclear protein in the human IgM gene. Nature. 1985; 314(6008):289-92. DOI: 10.1038/314289a0. View

20.

Boshart M, Weber F, Jahn G, Dorsch-Hasler K, Fleckenstein B, Schaffner W . A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. Cell. 1985; 41(2):521-30. DOI: 10.1016/s0092-8674(85)80025-8. View