Chagas Disease in Southern Coastal Ecuador: Coinfections with Arboviruses and a Comparison of Serological Assays for Chagas Disease Diagnosis

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2018 Oct 24

PMID 30350764

Citations 5

Authors

Neida K Mita-Mendoza

Elizabeth McMahon

Aileen Kenneson

Arturo Barbachano-Guerrero

Efrain Beltran-Ayala

Cinthya Cueva

Christine A King

Christina D Lupone

Yagahira E Castro-Sesquen

Robert H Gilman

Timothy P Endy

Anna M Stewart-Ibarra

Affiliations

Soon will be listed here.

Abstract

Occurrence of Chagas disease and arbovirus coinfections is unknown, despite the vast co-endemic areas throughout the Americas. This study examined the proportion of individuals positive for and coinfections with dengue, chikungunya, and Zika viruses in Machala, Ecuador (January 2014-December 2015). Chagas seropositivity was evaluated with five commercially available assays. Dengue infections were identified by nonstructural protein 1 rapid test and enzyme linked immunosorbent assay (ELISA), immunoglobulin M ELISA, and reverse transcription PCR (RT-PCR); chikungunya and Zika infections were identified by RT-PCR. Of 658 individuals, six were positive for (0.91%), including one /dengue coinfection and one /chikungunya/dengue coinfection. The clinical manifestations of coinfected individuals corresponded to severe dengue and dengue with warning signs, respectively. We observed discrepant results by using the Hemagen Chagas kit and the rapid test Chagas Detect Plus (false positives: 3.9% and 15.4%), highlighting the need to assess diagnostic assays in geographic regions with distinct taxonomic units of

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