Endodermal Pouch-expressed is Important for Pharyngeal Cartilage Formation

Pharyngeal pouches, a series of outpocketings derived from the foregut endoderm, are essential for craniofacial skeleton formation. However, the molecular mechanisms underlying endodermal pouch-regulated head cartilage development are not fully understood. In this study, we find that zebrafish , a gene encoding Doublesex- and Mab-3-related transcription factor, is specifically expressed in endodermal pouches and required for normal pharyngeal cartilage development. Loss of doesn't affect cranial neural crest (CNC) specification and migration, but leads to prechondrogenic condensation defects by reducing expression after CNC cell movement into the pharyngeal arches. Moreover, inactivation results in reduced proliferation and impaired differentiation of CNC cells. We also show that suppresses expression in endodermal pouches to maintain BMP/Smad signaling in the arches, thereby facilitating CNC cell proliferation and chondrogenic differentiation. This work provides insight into how transcription factors expressed in endodermal pouches regulate pharyngeal skeleton development through tissue-tissue interactions.