Assaying for Superoxide Dismutase Activity: Some Large Consequences of Minor Changes in Conditions

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1987 Mar 1

PMID 3034103

Citations 529

Authors

W F Beyer Jr

I Fridovich

Affiliations

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Abstract

Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.

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