Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites

Overview

Journal Open Access Maced J Med Sci

Specialty General Medicine

Date 2018 Oct 20

PMID 30337968

Citations 1

Authors

Nihal A Hanafy

Mohamed S Badr

Ghada M Nasr

Affiliations

Soon will be listed here.

Abstract

Background: is a common parasitic infection of humans. Infection is usually mild. Serious complications can occur in pregnant and immunocompromised patients.

Aim: The present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples.

Methods: The target DNA was provided in 8 different quantities.

Results: Amplification failure was reported only with the cPCR in samples of low concentrations using both primer sets. Quantitative PCR detected the 8 different dilutions of the purified using the 2 sets of primers while cPCR was sensitive to detect only 6 different dilutions.

Conclusion: Generally real-time quantitative molecular assays, is easy to use method compared to conventional PCR assay and produces more reliable results within only one hour time but still the possible application of qPCRs in routine diagnosis necessitates analysis of a large number of clinical samples in further studies to make the proper choice.

Citing Articles

Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children.

Hassan Z, Zekry K, Heikal E, Ibrahim H, Khirala S, Abd El-Hamid S Parasitol Res. 2023; 122(5):1177-1187.

PMID: 36917369 PMC: 10097734. DOI: 10.1007/s00436-023-07818-2.

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