Expression of Retrovirally Transduced Genes in Primary Cultures of Adult Rat Hepatocytes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 May 1

PMID 3033644

Citations 18

Authors

J A Wolff

J K Yee

H F Skelly

J C Moores

J G Respess

T Friedmann

H Leffert

Affiliations

Soon will be listed here.

Abstract

Differentiated primary rat hepatocyte cultures have been infected with retroviral vectors expressing human hypoxanthine/guanine phosphoribosyltransferase or the transposon Tn5 neomycin-resistance gene. Expression of the markers was detected only after infection of the cells during a short period of cell replication and transient dedifferentiation from days 1 to 5 of culture. Provirus integrated during that period remains fully expressed during the entire subsequent stationary period of culture up to at least 25 days. Selection with the neomycin analogue G418 of cells infected with the neomycin vector led to the appearance of cells with hepatocyte morphology in which newly synthesized albumin was detectable by immunoprecipitation, indicating successful infection of hepatocytes.

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