Comparison of the Conformation of an Oligonucleotide Containing a Central G-T Base Pair with the Non-mismatch Sequence by Proton NMR

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Apr 24

PMID 3033602

Citations 5

Authors

E Quignard

G V Fazakerley

G van der Marel

J H VAN Boom

W GUSCHLBAUER

Affiliations

Soon will be listed here.

Abstract

We have recorded NOESY spectra of two non-selfcomplementary undecanucleotide duplexes. From the observed NOEs we do not detect any significant distortion of the helix when a G-C pair is replaced by a G-T pair and the normal interresidue connectivities can be followed through the mismatch site. We conclude that the 2D spectra of the non-exchangeable protons do not allow differentiation between a wobble or rare tautomer form for the mismatch. NOE measurements in H2O, however, clearly show that the mismatch adopts a wobble structure and give information on the hydration in the minor groove for the G-T base pair which is embedded between two A-T base pairs in the sequence.

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