All Foot and Mouth Disease Virus Serotypes Initiate Protein Synthesis at Two Separate AUGs

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Apr 24

PMID 3033601

Citations 49

Authors

D V Sangar

S E Newton

D J Rowlands

B E Clarke

Affiliations

Soon will be listed here.

Abstract

Translation of the foot and mouth disease virus genome in vitro and in vivo indicated that all seven serotypes initiate protein synthesis at two separate AUGs. Sequence analysis of the region surrounding these AUGs has shown that the efficiency with which the initiating AUG is recognized is dependent on the flanking nucleotides. However, in vitro, the major factor determining which AUG is used is the concentration of Mg2+.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Pelham H, Jackson R . An efficient mRNA-dependent translation system from reticulocyte lysates. Eur J Biochem. 1976; 67(1):247-56. DOI: 10.1111/j.1432-1033.1976.tb10656.x. View

Rowlands D, Harris T, Brown F . More precise location of the polycytidylic acid tract in foot and mouth disease virus RNA. J Virol. 1978; 26(2):335-43. PMC: 354072. DOI: 10.1128/JVI.26.2.335-343.1978. View

Sangar D . The replication of picornaviruses. J Gen Virol. 1979; 45(1):1-13. DOI: 10.1099/0022-1317-45-1-1. View

Sangar D, Black D, Rowlands D, Harris T, Brown F . Location of the initiation site for protein synthesis on foot-and-mouth disease virus RNA by in vitro translation of defined fragments of the RNA. J Virol. 1980; 33(1):59-68. PMC: 288523. DOI: 10.1128/JVI.33.1.59-68.1980. View

Kozak M . Evaluation of the "scanning model" for initiation of protein synthesis in eucaryotes. Cell. 1980; 22(1 Pt 1):7-8. DOI: 10.1016/0092-8674(80)90148-8. View

Dorner A, Dorner L, Larsen G, Wimmer E, Anderson C . Identification of the initiation site of poliovirus polyprotein synthesis. J Virol. 1982; 42(3):1017-28. PMC: 256935. DOI: 10.1128/JVI.42.3.1017-1028.1982. View

Caton A, Brownlee G, Yewdell J, GERHARD W . The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell. 1982; 31(2 Pt 1):417-27. DOI: 10.1016/0092-8674(82)90135-0. View

Beck E, Forss S, Strebel K, Cattaneo R, Feil G . Structure of the FMDV translation initiation site and of the structural proteins. Nucleic Acids Res. 1983; 11(22):7873-85. PMC: 326546. DOI: 10.1093/nar/11.22.7873. View

10.

Kozak M . Selection of initiation sites by eucaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence for preproinsulin. Nucleic Acids Res. 1984; 12(9):3873-93. PMC: 318796. DOI: 10.1093/nar/12.9.3873. View

11.

Forss S, Strebel K, Beck E, Schaller H . Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucleic Acids Res. 1984; 12(16):6587-601. PMC: 320098. DOI: 10.1093/nar/12.16.6587. View

12.

Clarke B, Sangar D, Burroughs J, Newton S, Carroll A, Rowlands D . Two initiation sites for foot-and-mouth disease virus polyprotein in vivo. J Gen Virol. 1985; 66 ( Pt 12):2615-26. DOI: 10.1099/0022-1317-66-12-2615. View

13.

Kozak M . Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell. 1986; 44(2):283-92. DOI: 10.1016/0092-8674(86)90762-2. View

14.

Newton S, Carroll A, CAMPBELL R, Clarke B, Rowlands D . The sequence of foot-and-mouth disease virus RNA to the 5' side of the poly(C) tract. Gene. 1985; 40(2-3):331-6. DOI: 10.1016/0378-1119(85)90057-5. View

15.

Strebel K, Beck E . A second protease of foot-and-mouth disease virus. J Virol. 1986; 58(3):893-9. PMC: 252997. DOI: 10.1128/JVI.58.3.893-899.1986. View

16.

Kozak M . Influences of mRNA secondary structure on initiation by eukaryotic ribosomes. Proc Natl Acad Sci U S A. 1986; 83(9):2850-4. PMC: 323404. DOI: 10.1073/pnas.83.9.2850. View