Network Pharmacology-Based Validation of Caveolin-1 As a Key Mediator of Ai Du Qing Inhibition of Drug Resistance in Breast Cancer

Overview

Journal Front Pharmacol

Date 2018 Oct 19

PMID 30333750

Citations 14

Authors

Neng Wang

Bowen Yang

Xiaotong Zhang

Shengqi Wang

Yifeng Zheng

Xiong Li

Shan Liu

Hao Pan

Yingwei Li

Zhujuan Huang

Fengxue Zhang

Zhiyu Wang

Affiliations

Soon will be listed here.

Abstract

Chinese formulas have been paid increasing attention in cancer multidisciplinary therapy due to their multi-targets and multi-substances property. Here, we aim to investigate the anti-breast cancer and chemosensitizing function of Ai Du Qing (ADQ) formula made up of , , , and . Our findings revealed that ADQ significantly inhibited cell proliferation in both parental and chemo-resistant breast cancer cells, but with little cytotoxcity effects on the normal cells. Besides, ADQ was found to facilitate the G2/M arresting and apoptosis induction effects of paclitaxel. Network pharmacology and bioinformatics analysis further demonstrated that ADQ yielded 132 candidate compounds and 297 potential targets, and shared 22 putative targets associating with breast cancer chemoresponse. Enrichment analysis and experimental validation demonstrated that ADQ might improve breast cancer chemosensitivity inhibiting caveolin-1, which further triggered expression changes of cell cycle-related proteins p21/cyclinB1 and apoptosis-associated proteins PARP1, BAX and Bcl-2. Besides, ADQ enhanced paclitaxel chemosensitivity on breast cancer. Our study not only uncovers the novel function and mechanisms of ADQ in chemosensitizing breast cancer at least partly targeting caveolin-1, but also sheds novel light in utilizing network pharmacology in Chinese Medicine research.

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