Two Families of Repeated DNA Sequences in Thiobacillus Ferrooxidans

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 May 1

PMID 3032898

Citations 14

Authors

J R Yates

D S Holmes

Affiliations

Soon will be listed here.

Abstract

The genome of Thiobacillus ferrooxidans ATCC 19859 is about 2.8 X 10(6) base pairs as determined by analysis of reassociation kinetics of sheared DNA. This is 70% of the size of the genome of Escherichia coli. About 6% of the genome of T. ferrooxidans consists of moderately repetitive DNA sequences that are repeated an average of 20 times per genome. Two distinct repeated sequences, designated family 1 and family 2, have been analyzed in more detail. Both families are approximately 1 kilobase in length and are repeated 20 to 30 times per genome. Preliminary evidence from restriction enzyme analysis, Southern blotting experiments, and thermal melting analysis indicates that members of both families are conserved and are interspersed with single-copy DNA. Six copies of one family are present on the 45-kilobase-pair plasmid of strain ATCC 19859.

Citing Articles

Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains.

Moya-Beltran A, Gajdosik M, Rojas-Villalobos C, Beard S, Mandl M, Silva-Garcia D Sci Rep. 2023; 13(1):10876.

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Composition, Diversity and Functional Analysis of the Modern Microbiome of the Middle Triassic Cava Superiore Beds (Monte San Giorgio, Switzerland).

Arif S, Reitner J, Hoppert M Sci Rep. 2020; 9(1):20394.

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Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli.

Pal Roy M, Mazumdar D, Dutta S, Saha S, Ghosh S PLoS One. 2016; 11(1):e0145745.

PMID: 26808559 PMC: 4726635. DOI: 10.1371/journal.pone.0145745.

Growth and Maintenance of Thiobacillus ferrooxidans Cells.

Barron J, Lueking D Appl Environ Microbiol. 1990; 56(9):2801-6.

PMID: 16348288 PMC: 184846. DOI: 10.1128/aem.56.9.2801-2806.1990.

Identification of a gene cluster for the formation of extracellular polysaccharide precursors in the chemolithoautotroph Acidithiobacillus ferrooxidans.

Barreto M, Jedlicki E, Holmes D Appl Environ Microbiol. 2005; 71(6):2902-9.

PMID: 15932984 PMC: 1151869. DOI: 10.1128/AEM.71.6.2902-2909.2005.

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