Effect of Copper on Diesel Degradation in Pseudomonas Extremaustralis

Overview

Journal Extremophiles

Publisher Springer

Date 2018 Oct 18

PMID 30328541

Citations 3

Authors

Maria Antonela Colonnella

Leonardo Lizarraga

Leticia Rossi

Rocio Diaz Pena

Diego Egoburo

Nancy I Lopez

Laura J Raiger Iustman

Affiliations

Soon will be listed here.

Abstract

Environments co-contaminated with heavy metals and hydrocarbons have become an important problem worldwide, especially due to the effect of metals on hydrocarbon degrading microorganisms. Pseudomonas extremaustralis, a bacterium isolated from a pristine pond in Antarctica, showed high capabilities to cope with environmental stress and a very versatile metabolism that includes alkane degradation under microaerobic conditions. In this work, we analyzed P. extremaustralis' capability to resist high copper concentrations and the effect of copper presence in diesel biodegradation. We observed that P. extremaustralis resisted up to 4 mM CuSO in a rich medium such as LB. This copper resistance is sustained by the presence of the cus and cop operons together with other efflux systems and porins located in a single region in P. extremaustralis genome. When copper was present, diesel degradation was negatively affected, even though copper enhanced bacterial attachment to hydrocarbons. However, when a small amount of glucose (0.05% w/v) was added, the presence of CuSO enhanced alkane degradation. In addition, atomic force microscopy analysis showed that the presence of glucose decreased the negative effects produced by copper and diesel on the cell envelopes.

Citing Articles

Ecological features of trace elements tolerant microbes isolated from sewage sludge of urban wastewater treatment plant.

Perelomov L, Rajput V, Gertsen M, Sizova O, Perelomova I, Kozmenko S Stress Biol. 2024; 4(1):8.

PMID: 38273092 PMC: 10810767. DOI: 10.1007/s44154-023-00144-8.

Isolation of Novel Bacterial Strains CSW01 and CSW02 from Sewage Sludge for Paracetamol Biodegradation.

Vargas-Ordonez A, Aguilar-Romero I, Villaverde J, Madrid F, Morillo E Microorganisms. 2023; 11(1).

PMID: 36677487 PMC: 9865377. DOI: 10.3390/microorganisms11010196.

Assessing the Involvement of Selected Phenotypes of PICF7 in Olive Root Colonization and Biological Control of .

Montes-Osuna N, Gomez-Lama Cabanas C, Valverde-Corredor A, Berendsen R, Prieto P, Mercado-Blanco J Plants (Basel). 2021; 10(2).

PMID: 33672351 PMC: 7926765. DOI: 10.3390/plants10020412.

References

Chayabutra C, Ju L . Degradation of n-hexadecane and its metabolites by Pseudomonas aeruginosa under microaerobic and anaerobic denitrifying conditions. Appl Environ Microbiol. 2000; 66(2):493-8. PMC: 91854. DOI: 10.1128/AEM.66.2.493-498.2000. View

Arnoldi M, Fritz M, Bauerlein E, Radmacher M, Sackmann E, Boulbitch A . Bacterial turgor pressure can be measured by atomic force microscopy. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 2000; 62(1 Pt B):1034-44. DOI: 10.1103/physreve.62.1034. View

Sani R, Peyton B, BROWN L . Copper-induced inhibition of growth of Desulfovibrio desulfuricans G20: assessment of its toxicity and correlation with those of zinc and lead. Appl Environ Microbiol. 2001; 67(10):4765-72. PMC: 93230. DOI: 10.1128/AEM.67.10.4765-4772.2001. View

Lee S, Grass G, Rensing C, Barrett S, Yates C, Stoyanov J . The Pco proteins are involved in periplasmic copper handling in Escherichia coli. Biochem Biophys Res Commun. 2002; 295(3):616-20. DOI: 10.1016/s0006-291x(02)00726-x. View

Norman R, Frontera-Suau R, Morris P . Variability in Pseudomonas aeruginosa lipopolysaccharide expression during crude oil degradation. Appl Environ Microbiol. 2002; 68(10):5096-103. PMC: 126393. DOI: 10.1128/AEM.68.10.5096-5103.2002. View

Ayub N, Pettinari M, Ruiz J, Lopez N . A polyhydroxybutyrate-producing Pseudomonas sp. isolated from Antarctic environments with high stress resistance. Curr Microbiol. 2004; 49(3):170-4. DOI: 10.1007/s00284-004-4254-2. View

Overbeek R, Begley T, Butler R, Choudhuri J, Chuang H, Cohoon M . The subsystems approach to genome annotation and its use in the project to annotate 1000 genomes. Nucleic Acids Res. 2005; 33(17):5691-702. PMC: 1251668. DOI: 10.1093/nar/gki866. View

Lageveen R, Huisman G, Preusting H, Ketelaar P, Eggink G, Witholt B . Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates. Appl Environ Microbiol. 1988; 54(12):2924-32. PMC: 204405. DOI: 10.1128/aem.54.12.2924-2932.1988. View

Basu A, Apte S, Phale P . Preferential utilization of aromatic compounds over glucose by Pseudomonas putida CSV86. Appl Environ Microbiol. 2006; 72(3):2226-30. PMC: 1393237. DOI: 10.1128/AEM.72.3.2226-2230.2006. View

10.

Bai W, Zhao K, Asami K . Effects of copper on dielectric properties of E. coli cells. Colloids Surf B Biointerfaces. 2007; 58(2):105-15. DOI: 10.1016/j.colsurfb.2007.02.015. View

11.

Basu A, Das D, Bapat P, Wangikar P, Phale P . Sequential utilization of substrates by Pseudomonas putida CSV86: signatures of intermediate metabolites and online measurements. Microbiol Res. 2007; 164(4):429-37. DOI: 10.1016/j.micres.2007.02.008. View

12.

Vullo D, Ceretti H, Daniel M, Ramirez S, Zalts A . Cadmium, zinc and copper biosorption mediated by Pseudomonas veronii 2E. Bioresour Technol. 2007; 99(13):5574-81. DOI: 10.1016/j.biortech.2007.10.060. View

13.

Gaboriaud F, Gee M, Strugnell R, Duval J . Coupled electrostatic, hydrodynamic, and mechanical properties of bacterial interfaces in aqueous media. Langmuir. 2008; 24(19):10988-95. DOI: 10.1021/la800258n. View

14.

Lopez N, Pettinari M, Stackebrandt E, Tribelli P, Potter M, Steinbuchel A . Pseudomonas extremaustralis sp. nov., a Poly(3-hydroxybutyrate) producer isolated from an antarctic environment. Curr Microbiol. 2009; 59(5):514-9. DOI: 10.1007/s00284-009-9469-9. View

15.

Rojo F . Carbon catabolite repression in Pseudomonas : optimizing metabolic versatility and interactions with the environment. FEMS Microbiol Rev. 2010; 34(5):658-84. DOI: 10.1111/j.1574-6976.2010.00218.x. View

16.

Mykytczuk N, Trevors J, Ferroni G, Leduc L . Cytoplasmic membrane response to copper and nickel in Acidithiobacillus ferrooxidans. Microbiol Res. 2010; 166(3):186-206. DOI: 10.1016/j.micres.2010.03.004. View

17.

Francius G, Polyakov P, Merlin J, Abe Y, Ghigo J, Merlin C . Bacterial surface appendages strongly impact nanomechanical and electrokinetic properties of Escherichia coli cells subjected to osmotic stress. PLoS One. 2011; 6(5):e20066. PMC: 3105017. DOI: 10.1371/journal.pone.0020066. View

18.

Tribelli P, Di Martino C, Lopez N, Raiger Iustman L . Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis. Biodegradation. 2012; 23(5):645-51. DOI: 10.1007/s10532-012-9540-2. View

19.

Tribelli P, Raiger Iustman L, Catone M, Di Martino C, Revale S, Mendez B . Genome sequence of the polyhydroxybutyrate producer Pseudomonas extremaustralis, a highly stress-resistant Antarctic bacterium. J Bacteriol. 2012; 194(9):2381-2. PMC: 3347083. DOI: 10.1128/JB.00172-12. View

20.

Kaczorek E, Salek K, Guzik U, Jesionowski T, Cybulski Z . Biodegradation of alkyl derivatives of aromatic hydrocarbons and cell surface properties of a strain of Pseudomonas stutzeri. Chemosphere. 2012; 90(2):471-8. DOI: 10.1016/j.chemosphere.2012.07.065. View