Complete Genome Sequence of the Model Halovirus PhiH1 (ΦH1)
Overview
Affiliations
The halophilic myohalovirus (ΦH) was first described in 1982 and was isolated from a spontaneously lysed culture of strain R1. Until 1994, it was used extensively as a model to study the molecular genetics of haloarchaea, but only parts of the viral genome were sequenced during this period. Using Sanger sequencing combined with high-coverage Illumina sequencing, the full genome sequence of the major variant (phiH1) of this halovirus has been determined. The dsDNA genome is 58,072 bp in length and carries 97 protein-coding genes. We have integrated this information with the previously described transcription mapping data. PhiH could be classified into Myoviridae Type1, Cluster 4 based on capsid assembly and structural proteins (VIRFAM). The closest relative was virus phiCh1 (φCh1), which shared 63% nucleotide identity and displayed a high level of gene synteny. This close relationship was supported by phylogenetic tree reconstructions. The complete sequence of this historically important virus will allow its inclusion in studies of comparative genomics and virus diversity.
Revisiting evolutionary trajectories and the organization of the Pleolipoviridae family.
Alarcon-Schumacher T, Lucking D, Erdmann S PLoS Genet. 2023; 19(10):e1010998.
PMID: 37831715 PMC: 10599561. DOI: 10.1371/journal.pgen.1010998.
Alarcon-Schumacher T, Naor A, Gophna U, Erdmann S Proc Natl Acad Sci U S A. 2022; 119(35):e2205037119.
PMID: 35994644 PMC: 9436352. DOI: 10.1073/pnas.2205037119.
Halovirus HF2 Intergenic Repeat Sequences Carry Promoters.
Russ B, Pfeiffer F, Dyall-Smith M Viruses. 2021; 13(12).
PMID: 34960657 PMC: 8707807. DOI: 10.3390/v13122388.
Diversity, taxonomy, and evolution of archaeal viruses of the class Caudoviricetes.
Liu Y, Demina T, Roux S, Aiewsakun P, Kazlauskas D, Simmonds P PLoS Biol. 2021; 19(11):e3001442.
PMID: 34752450 PMC: 8651126. DOI: 10.1371/journal.pbio.3001442.
The Novel Halovirus Hardycor1, and the Presence of Active (Induced) Proviruses in Four Haloarchaea.
Dyall-Smith M, Pfeiffer F, Chiang P, Tang S Genes (Basel). 2021; 12(2).
PMID: 33498646 PMC: 7911831. DOI: 10.3390/genes12020149.