Assembly of Protein Complexes Restricts Diffusion of Wnt3a Proteins

Overview

Journal Commun Biol

Specialty Biology

Date 2018 Oct 16

PMID 30320232

Citations 15

Authors

Ritsuko Takada

Yusuke Mii

Elena Krayukhina

Yuusuke Maruyama

Kazuhiro Mio

Yoshikazu Sasaki

Takao Shinkawa

Chan-Gi Pack

Yasushi Sako

Chikara Sato

Susumu Uchiyama

Shinji Takada

Affiliations

Soon will be listed here.

Abstract

Members of the Wnt protein family play roles in many aspects of embryogenesis and homeostasis. Despite their biological significance, characteristics of Wnt proteins still remain unclear, mainly due to their insolubility after the removal of serum. Here we examine Wnt proteins in serum-containing media by using analytical ultracentrifugation with a fluorescence detection system. This analysis reveals that Wnt3a assembles into high-molecular-weight complexes that become dissociable by interaction with the extracellular domain of the Frizzled8 receptor or secreted Wnt-binding protein sFRP2. Cross-linking and single-particle analyses of Wnt3a fractionated by gel filtration chromatography show the homo-trimer to be the smallest form of the assembled Wnt3a complexes. Fluorescence correlation spectroscopy and immunohistochemistry reveal that the assembly of Wnt3a complexes restricted their diffusion and signaling range in embryos. Thus, we propose that the Wnt diffusion range can be controlled by a balance between the assembly of Wnt complexes and their dissociation.

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