Central Dicer-miR-103/107 Controls Developmental Switch of POMC Progenitors into NPY Neurons and Impacts Glucose Homeostasis

Overview

Journal Elife

Specialty Biology

Date 2018 Oct 13

PMID 30311908

Citations 21

Authors

Sophie Croizier

Soyoung Park

Julien Maillard

Sebastien G Bouret

Affiliations

Soon will be listed here.

Abstract

Proopiomelanocortin (POMC) neurons are major negative regulators of energy balance. A distinct developmental property of POMC neurons is that they can adopt an orexigenic neuropeptide Y (NPY) phenotype. However, the mechanisms underlying the differentiation of progenitors remain unknown. Here, we show that the loss of the microRNA (miRNA)-processing enzyme in POMC neurons causes metabolic defects, an age-dependent decline in the number of mRNA-expressing cells, and an increased proportion of progenitors acquiring a NPY phenotype. miRNome microarray screening further identified miR-103/107 as candidates that may be involved in the maturation of progenitors. In vitro inhibition of miR-103/107 causes a reduction in the number of -expressing cells and increases the proportion of progenitors differentiating into NPY neurons. Moreover, in utero silencing of miR-103/107 causes perturbations in glucose homeostasis. Together, these data suggest a role for prenatal miR-103/107 in the maturation of progenitors and glucose homeostasis.

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