Carboxymethyl Chitosan Nanoparticles Loaded with Bioactive Peptide OH-CATH30 Benefit Nonscar Wound Healing

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2018 Oct 13

PMID 30310280

Citations 15

Authors

Tongyi Sun

Bo Zhan

Weifen Zhang

Di Qin

Guixue Xia

Huijie Zhang

Meiyu Peng

Sheng-An Li

Yun Zhang

Yuanyuan Gao

Wen-Hui Lee

Affiliations

Soon will be listed here.

Abstract

Background: Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide. Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.

Purpose: To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.

Methods: CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering. Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC. Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods. The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.

Results: The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner. CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes. In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone. Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition. CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.

Conclusion: The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.

Citing Articles

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Medical Applications and Cellular Mechanisms of Action of Carboxymethyl Chitosan Hydrogels.

Kruczkowska W, Klosinski K, Grabowska K, Galeziewska J, Gromek P, Kciuk M Molecules. 2024; 29(18).

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Ross A, Sauce-Guevara M, Alarcon E, Mendez-Rojas M Front Bioeng Biotechnol. 2022; 10:893936.

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