Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex

Overview

Journal Cereb Cortex

Publisher Oxford University Press

Specialty Neurology

Date 2018 Oct 12

PMID 30307479

Citations 8

Authors

Keiko Nakanishi

Hiroyuki Niida

Hidenori Tabata

Tsuyoshi Ito

Yuki Hori

Madoka Hattori

Yoshikazu Johmura

Chisato Yamada

Takashi Ueda

Kosei Takeuchi

Kenichiro Yamada

Koh-Ichi Nagata

Nobuaki Wakamatsu

Masashi Kishi

Y Albert Pan

Shinya Ugawa

Shoichi Shimada

Joshua R Sanes

Yujiro Higashi

Makoto Nakanishi

Affiliations

Soon will be listed here.

Abstract

SAD kinases regulate presynaptic vesicle clustering and neuronal polarization. A previous report demonstrated that Sada-/- and Sadb-/- double-mutant mice showed perinatal lethality with a severe defect in axon/dendrite differentiation, but their single mutants did not. These results indicated that they were functionally redundant. Surprisingly, we show that on a C57BL/6N background, SAD-A is essential for cortical development whereas SAD-B is dispensable. Sada-/- mice died within a few days after birth. Their cortical lamination pattern was disorganized and radial migration of cortical neurons was perturbed. Birth date analyses with BrdU and in utero electroporation using pCAG-EGFP vector showed a delayed migration of cortical neurons to the pial surface in Sada-/- mice. Time-lapse imaging of these mice confirmed slow migration velocity in the cortical plate. While the neurites of hippocampal neurons in Sada-/- mice could ultimately differentiate in culture to form axons and dendrites, the average length of their axons was shorter than that of the wild type. Thus, analysis on a different genetic background than that used initially revealed a nonredundant role for SAD-A in neuronal migration and differentiation.

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