Reactivity of Modified Ribose Moieties of Guanosine: New Cleavage Reactions Mediated by the IVS of Tetrahymena Precursor RRNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Feb 25

PMID 3029719

Citations 4

Authors

P S Kay

T Inoue

Affiliations

Soon will be listed here.

Abstract

An RNA molecule consisting of the 5' exon and intervening sequence (IVS) of Tetrahymena precursor rRNA was oxidized with sodium periodate to convert the ribose moiety of the 3' terminal guanosine into a dialdehyde form. The modified RNA undergoes a specific cleavage reaction at the 5' splice site, but has no apparent cyclization activity. This novel reaction mediated by the IVS RNA is pH dependent over the range 6.5-8.5 and leaves a 5' phosphate and a 3'-OH at the newly created termini. The dialdehyde form of monomer guanosine is also capable of causing a specific cleavage reaction at the 5' splice site although the nucleotide is not covalently attached to the IVS RNA in the final product. These and other findings described in this report suggest that the cis diol of the intact ribose moiety of guanosine is not an absolute requirement for the IVS-mediated reactions.

Citing Articles

Two guanosine binding sites exist in group I self-splicing IVS RNAs.

Kay P, Menzel P, Inoue T EMBO J. 1988; 7(11):3531-7.

PMID: 3208741 PMC: 454854. DOI: 10.1002/j.1460-2075.1988.tb03229.x.

Identification of phosphate groups important to self-splicing of the Tetrahymena rRNA intron as determined by phosphorothioate substitution.

Waring R Nucleic Acids Res. 1989; 17(24):10281-93.

PMID: 2690016 PMC: 335300. DOI: 10.1093/nar/17.24.10281.

The relationship between RNA catalytic processes.

Cedergren R, Lang B, Gravel D Orig Life Evol Biosph. 1988; 18(3):299-305.

PMID: 2465524 DOI: 10.1007/BF01804676.

Selection of small molecules by the Tetrahymena catalytic center.

Yarus M, Illangesekare M, Christian E Nucleic Acids Res. 1991; 19(6):1297-304.

PMID: 2030946 PMC: 333857. DOI: 10.1093/nar/19.6.1297.

References

Donis-Keller H, Maxam A, Gilbert W . Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977; 4(8):2527-38. PMC: 342589. DOI: 10.1093/nar/4.8.2527. View

Schwartz A, Orgel L . Template-directed synthesis of novel, nucleic acid-like structures. Science. 1985; 228:585-7. DOI: 10.1126/science.228.4699.585. View

Davies R, Waring R, Ray J, Brown T, Scazzocchio C . Making ends meet: a model for RNA splicing in fungal mitochondria. Nature. 1982; 300(5894):719-24. DOI: 10.1038/300719a0. View

Michel F, Jacquier A, Dujon B . Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure. Biochimie. 1982; 64(10):867-81. DOI: 10.1016/s0300-9084(82)80349-0. View

Kruger K, Grabowski P, Zaug A, Sands J, Gottschling D, Cech T . Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell. 1982; 31(1):147-57. DOI: 10.1016/0092-8674(82)90414-7. View

Zaug A, Grabowski P, Cech T . Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage-ligation reaction. Nature. 1983; 301(5901):578-83. DOI: 10.1038/301578a0. View

Bass B, Cech T . Specific interaction between the self-splicing RNA of Tetrahymena and its guanosine substrate: implications for biological catalysis by RNA. Nature. 1984; 308(5962):820-6. DOI: 10.1038/308820a0. View

Zielinski W, Orgel L . Oligomerization of activated derivatives of 3'-amino-3'-deoxyguanosine on poly(C) and poly(dC) templates. Nucleic Acids Res. 1985; 13(7):2469-84. PMC: 341169. DOI: 10.1093/nar/13.7.2469. View

Marsh T, Pace N . Ribonuclease P catalysis differs from ribosomal RNA self-splicing. Science. 1985; 229(4708):79-81. DOI: 10.1126/science.4012313. View

10.

Sullivan F, Cech T . Reversibility of cyclization of the Tetrahymena rRNA intervening sequence: implication for the mechanism of splice site choice. Cell. 1985; 42(2):639-48. DOI: 10.1016/0092-8674(85)90121-7. View

11.

Zaug A, Cech T . The intervening sequence RNA of Tetrahymena is an enzyme. Science. 1986; 231(4737):470-5. DOI: 10.1126/science.3941911. View

12.

Zaug A, Kent J, Cech T . Reactions of the intervening sequence of the Tetrahymena ribosomal ribonucleic acid precursor: pH dependence of cyclization and site-specific hydrolysis. Biochemistry. 1985; 24(22):6211-8. DOI: 10.1021/bi00343a027. View

13.

Cech T . The generality of self-splicing RNA: relationship to nuclear mRNA splicing. Cell. 1986; 44(2):207-10. DOI: 10.1016/0092-8674(86)90751-8. View

14.

Zaug A, Cech T . The Tetrahymena intervening sequence ribonucleic acid enzyme is a phosphotransferase and an acid phosphatase. Biochemistry. 1986; 25(16):4478-82. DOI: 10.1021/bi00364a002. View

15.

Been M, Cech T . One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity. Cell. 1986; 47(2):207-16. DOI: 10.1016/0092-8674(86)90443-5. View

16.

Inoue T, Sullivan F, Cech T . New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicing. J Mol Biol. 1986; 189(1):143-65. DOI: 10.1016/0022-2836(86)90387-6. View

17.

Zaug A, Been M, Cech T . The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature. 1986; 324(6096):429-33. DOI: 10.1038/324429a0. View

18.

Winter G, Brownlee G . 3'End labelling of RNA with 32P suitable for rapid gel sequencing. Nucleic Acids Res. 1978; 5(9):3129-39. PMC: 342236. DOI: 10.1093/nar/5.9.3129. View