Nucleotide Sequence and Expression of the Gene Encoding the EcoRII Modification Enzyme

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Jan 12

PMID 3029675

Citations 50

Authors

S Som

A S Bhagwat

S Friedman

Affiliations

Soon will be listed here.

Abstract

The gene coding for the EcoRII modification enzyme has been cloned and the nucleotide sequence of 1933 base pairs containing the gene has been determined. The gene codes for a protein of 477 amino acids. Two transcriptional start sites have been mapped by S1 mapping. One deletion that removes 34 N-terminal amino acids was found to have partial enzyme activity. Comparison of the EcoRII methylase sequence with other cytosine methylases revealed several domains of partial homology among all cytosine methylases. Cloning the gene in multicopy pUC vectors increased the expression by 6-18 fold. A 40 fold overproduction of the EcoRII methylase was obtained by cloning the gene in the expression vector carrying the lambda PL promoter.

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References

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

Hattman S, Schlagman S, Cousens L . Isolation of a mutant of Escherichia coli defective in cytosine-specific deoxyribonucleic acid methylase activity and in partial protection of bacteriophage lambda against restriction by cells containing the N-3 drug-resistance factor. J Bacteriol. 1973; 115(3):1103-7. PMC: 246359. DOI: 10.1128/jb.115.3.1103-1107.1973. View

May M, Hattaman S . Deoxyribonucleic acid-cytosine methylation by host- and plasmid-controlled enzymes. J Bacteriol. 1975; 122(1):129-38. PMC: 235649. DOI: 10.1128/jb.122.1.129-138.1975. View

SANGER F, Air G, Barrell B, Brown N, Coulson A, Fiddes C . Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977; 265(5596):687-95. DOI: 10.1038/265687a0. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Kosykh V, Buryanov Y, Bayev A . Molecular cloning of EcoRII endonuclease and methylase genes. Mol Gen Genet. 1980; 178(3):717-8. DOI: 10.1007/BF00337884. View

10.

GOTTESMAN M, Adhya S, Das A . Transcription antitermination by bacteriophage lambda N gene product. J Mol Biol. 1980; 140(1):57-75. DOI: 10.1016/0022-2836(80)90356-3. View

11.

Newman A, Rubin R, Kim S, Modrich P . DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes. J Biol Chem. 1981; 256(5):2131-9. View

12.

Greene P, Gupta M, Boyer H, Brown W, Rosenberg J . Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase. J Biol Chem. 1981; 256(5):2143-53. View

13.

Grantham R, Gautier C, Gouy M, Jacobzone M, Mercier R . Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981; 9(1):r43-74. PMC: 326682. DOI: 10.1093/nar/9.1.213-b. View

14.

Burianov I, Nesterenko V, Kosykh V, Baev A . [Differing molecular structures of EcoRII and Eco MRE600 dcmII DNA methylases]. Dokl Akad Nauk SSSR. 1981; 257(2):495-7. View

15.

Friedman S . The inhibition of DNA(cytosine-5)methylases by 5-azacytidine. The effect of azacytosine-containing DNA. Mol Pharmacol. 1981; 19(2):314-20. View

16.

GOAD W, Kanehisa M . Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries. Nucleic Acids Res. 1982; 10(1):247-63. PMC: 326131. DOI: 10.1093/nar/10.1.247. View

17.

Delaney A . A DNA sequence handling program. Nucleic Acids Res. 1982; 10(1):61-7. PMC: 326114. DOI: 10.1093/nar/10.1.61. View

18.

Friedman S . Bactericidal effect of 5-azacytidine on Escherichia coli carrying EcoRII restriction-modification enzymes. J Bacteriol. 1982; 151(1):262-8. PMC: 220236. DOI: 10.1128/jb.151.1.262-268.1982. View

19.

Brosius J, Cate R, Perlmutter A . Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro. J Biol Chem. 1982; 257(15):9205-10. View

20.

Staden R . An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Res. 1982; 10(9):2951-61. PMC: 320667. DOI: 10.1093/nar/10.9.2951. View