Immunomodulatory Activity of a Novel Polysaccharide from Lonicera Japonica in Immunosuppressed Mice Induced by Cyclophosphamide

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2018 Oct 9

PMID 30296293

Citations 40

Authors

Xiaonan Zhou

Qun Dong

Xianzhao Kan

Lihong Peng

Xingyu Xu

Yun Fang

Jialiang Yang

Affiliations

Soon will be listed here.

Abstract

Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.

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