Brain Metabolism Under Different Anesthetic Conditions Using Hyperpolarized [1- C]pyruvate and [2- C]pyruvate

Overview

Journal NMR Biomed

Publisher Wiley

Date 2018 Oct 3

PMID 30276897

Citations 10

Authors

Malgorzata Marjanska

Alexander A Shestov

Dinesh K Deelchand

Emily Kittelson

Pierre-Gilles Henry

Affiliations

Soon will be listed here.

Abstract

Carbon-13 NMR spectroscopy ( C MRS) offers the unique capability to measure brain metabolic rates in vivo. Hyperpolarized C reduces the time required to assess brain metabolism from hours to minutes when compared with conventional C MRS. This study investigates metabolism of hyperpolarized [1- C]pyruvate and [2- C]pyruvate in the rat brain in vivo under various anesthetics: pentobarbital, isoflurane, α-chloralose, and morphine. The apparent metabolic rate from pyruvate to lactate modeled using time courses obtained after injection of hyperpolarized [1- C]pyruvate was significantly greater for isoflurane than for all other anesthetic conditions, and significantly greater for morphine than for α-chloralose. The apparent metabolic rate from pyruvate to bicarbonate was significantly greater for morphine than for all other anesthetic conditions, and significantly lower for pentobarbital than for α-chloralose. Results show that relative TCA cycle rates determined from hyperpolarized C data are consistent with rates previously measured using conventional C MRS under similar anesthetic conditions, and that using morphine for sedation greatly improves detection of downstream metabolic products compared with other anesthetics.

Citing Articles

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Probing Cerebral Metabolism with Hyperpolarized C Imaging after Opening the Blood-Brain Barrier with Focused Ultrasound.

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