The Role of MiR-106p-5p in Cervical Cancer: from Expression to Molecular Mechanism

Overview

Journal Cell Death Discov

Date 2018 Oct 3

PMID 30275981

Citations 13

Authors

Yuexiong Yi

Yanyan Liu

Wanrong Wu

Kejia Wu

Wei Zhang

Affiliations

Soon will be listed here.

Abstract

This study aims to investigate the role of miR-106b-5p in cervical cancer by performing a comprehensive analysis on its expression and identifying its putative molecular targets and pathways based on The Cancer Genome Atlas (TCGA) dataset, Gene Expression Omnibus (GEO) dataset, and literature review. Significant upregulation of miR-106b-5p in cervical cancer is confirmed by meta-analysis with the data from TCGA, GEO, and literature. Moreover, the expression of miR-106b-5p is significantly correlated with the number of metastatic lymph nodes. Our bioinformatics analyses show that miR-106b could promote cervical cancer progression by modulating the expression of GSK3B, VEGFA, and PTK2 genes. Importantly, these three genes play a crucial role in PI3K-Akt signaling, focal adhesion, and cancer. Both the expression of miR-106b-5p and key genes are upregulated in cervical cancer. Several explanations could be implemented for this upregulation. However, the specific mechanism needs to be investigated further.

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References

Jiang H, Hegde S, Knolhoff B, Zhu Y, Herndon J, Meyer M . Targeting focal adhesion kinase renders pancreatic cancers responsive to checkpoint immunotherapy. Nat Med. 2016; 22(8):851-60. PMC: 4935930. DOI: 10.1038/nm.4123. View

Wang T, Xu H, Qi M, Yan S, Tian X . miRNA dysregulation and the risk of metastasis and invasion in papillary thyroid cancer: a systematic review and meta-analysis. Oncotarget. 2018; 9(4):5473-5479. PMC: 5797065. DOI: 10.18632/oncotarget.16681. View

Ma C, Zeng C, Jin L, Yang Y, Li P, Chen L . GSK3β mediates the carcinogenic effect of HPV16 in cervical cancer. Sci Rep. 2015; 5:16555. PMC: 4642308. DOI: 10.1038/srep16555. View

Liu F, Zhang S, Zhao Z, Mao X, Huang J, Wu Z . MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer. Oncotarget. 2016; 7(15):19666-79. PMC: 4991410. DOI: 10.18632/oncotarget.7531. View

ODonnell K, Wentzel E, Zeller K, Dang C, Mendell J . c-Myc-regulated microRNAs modulate E2F1 expression. Nature. 2005; 435(7043):839-43. DOI: 10.1038/nature03677. View

Ma K, Fu W, Tang M, Zhang C, Hou T, Li R . PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment. Autophagy. 2017; 13(3):579-591. PMC: 5361600. DOI: 10.1080/15548627.2016.1272742. View

Truesdell S, Mortensen R, Seo M, Schroeder J, Lee J, LeTonqueze O . MicroRNA-mediated mRNA translation activation in quiescent cells and oocytes involves recruitment of a nuclear microRNP. Sci Rep. 2012; 2:842. PMC: 3496365. DOI: 10.1038/srep00842. View

Siegel R, Miller K, Jemal A . Cancer statistics, 2018. CA Cancer J Clin. 2018; 68(1):7-30. DOI: 10.3322/caac.21442. View

Cheng Y, Guo Y, Zhang Y, You K, Li Z, Geng L . MicroRNA-106b is involved in transforming growth factor β1-induced cell migration by targeting disabled homolog 2 in cervical carcinoma. J Exp Clin Cancer Res. 2016; 35:11. PMC: 4714510. DOI: 10.1186/s13046-016-0290-6. View

10.

Kim V . Small RNAs: classification, biogenesis, and function. Mol Cells. 2005; 19(1):1-15. View

11.

Liang J, Slingerland J . Multiple roles of the PI3K/PKB (Akt) pathway in cell cycle progression. Cell Cycle. 2003; 2(4):339-45. View

12.

Catalanotto C, Cogoni C, Zardo G . MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions. Int J Mol Sci. 2016; 17(10). PMC: 5085744. DOI: 10.3390/ijms17101712. View

13.

Brazma A, Hingamp P, Quackenbush J, Sherlock G, Spellman P, Stoeckert C . Minimum information about a microarray experiment (MIAME)-toward standards for microarray data. Nat Genet. 2001; 29(4):365-71. DOI: 10.1038/ng1201-365. View

14.

Wahid F, Shehzad A, Khan T, Kim Y . MicroRNAs: synthesis, mechanism, function, and recent clinical trials. Biochim Biophys Acta. 2010; 1803(11):1231-43. DOI: 10.1016/j.bbamcr.2010.06.013. View

15.

Petry K . HPV and cervical cancer. Scand J Clin Lab Invest Suppl. 2014; 244:59-62. DOI: 10.3109/00365513.2014.936683. View

16.

Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J . STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res. 2014; 43(Database issue):D447-52. PMC: 4383874. DOI: 10.1093/nar/gku1003. View

17.

Conway J, Lex A, Gehlenborg N . UpSetR: an R package for the visualization of intersecting sets and their properties. Bioinformatics. 2017; 33(18):2938-2940. PMC: 5870712. DOI: 10.1093/bioinformatics/btx364. View

18.

Xiang W, He J, Huang C, Chen L, Tao D, Wu X . miR-106b-5p targets tumor suppressor gene SETD2 to inactive its function in clear cell renal cell carcinoma. Oncotarget. 2015; 6(6):4066-79. PMC: 4414173. DOI: 10.18632/oncotarget.2926. View

19.

Piao J, You K, Guo Y, Zhang Y, Li Z, Geng L . Substrate stiffness affects epithelial-mesenchymal transition of cervical cancer cells through miR-106b and its target protein DAB2. Int J Oncol. 2017; 50(6):2033-2042. DOI: 10.3892/ijo.2017.3978. View

20.

Kozomara A, Griffiths-Jones S . miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res. 2013; 42(Database issue):D68-73. PMC: 3965103. DOI: 10.1093/nar/gkt1181. View