Expression of Mink Cell Focus-forming Murine Leukemia Virus-related Transcripts in AKR Mice

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1987 Mar 1

PMID 3027414

Citations 14

Authors

A S Khan

F Laigret

C P Rodi

Affiliations

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Abstract

We used a synthetic 16-base-pair mink cell focus-forming (MCF) env-specific oligomer as radiolabeled probe to study MCF murine leukemia virus (MuLV)-related transcripts in brain, kidney, liver, spleen, and thymus tissues of AKR mice ranging from 5 weeks to 6 months (mo) of age. Tissue-specific expression of poly (A) + RNAs was seen: 6.0-kilobase (kb) transcripts were detected in the liver and kidney; 7.2- and 1.8-kb RNA species were present in the thymus. In addition, all the tissues tested contained 3.0-kb messages. The transcription of these MCF-related mRNAs was independent of the presence of ecotropic and xenotropic MuLVs. In general, expression of the MCF env-related transcripts appeared to peak at 2 mo of age; these messages were barely detectable in brain, kidney, liver, and spleen tissues after 2 mo and in thymus tissue after 4 mo of age. All of the subgenomic MCF env-related mRNAs (6.0, 7.2, 1.8, and 3.0 kb) appeared to contain the 190-base-pair cellular DNA insert, characteristic of the long terminal repeats associated with endogenous MCF env-related proviruses (A. S. Khan and M. A. Martin, Proc. Natl. Acad. Sci. USA 80:2699-2703, 1983). No genomic-size (8.4-kb) transcripts corresponding to endogenous MCF-related proviruses were detected. An 8.4-kb MCF env-related mRNA was first seen at 3 mo of age, exclusively in thymus tissue. This species most likely represents the first appearance of a recombinant MCF-related MuLV genome. The transcripts which were detected in thymus tissue might be involved in the generation of leukemogenic MCF viruses.

Citing Articles

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Structure, distribution, and expression of an ancient murine endogenous retroviruslike DNA family.

Obata M, Khan A J Virol. 1988; 62(11):4381-6.

PMID: 3172346 PMC: 253879. DOI: 10.1128/JVI.62.11.4381-4386.1988.

Multiple endogenous xenotropic and mink cell focus-forming murine leukemia virus-related transcripts are induced by polyclonal immune activators.

Krieg A, Khan A, Steinberg A J Virol. 1988; 62(10):3545-50.

PMID: 2843657 PMC: 253492. DOI: 10.1128/JVI.62.10.3545-3550.1988.

Potential progenitor sequences of mink cell focus-forming (MCF) murine leukemia viruses: ecotropic, xenotropic, and MCF-related viral RNAs are detected concurrently in thymus tissues of AKR mice.

Laigret F, Repaske R, Boulukos K, Rabson A, Khan A J Virol. 1988; 62(2):376-86.

PMID: 2826802 PMC: 250546. DOI: 10.1128/JVI.62.2.376-386.1988.

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