Vascular Extracellular Matrix and Fibroblasts-coculture Directed Differentiation of Human Mesenchymal Stem Cells Toward Smooth Muscle-like Cells for Vascular Tissue Engineering

Overview

Journal Mater Sci Eng C Mater Biol Appl

Publisher Elsevier

Specialties Biomedical Engineering
Biotechnology

Date 2018 Oct 3

PMID 30274093

Citations 14

Authors

Na Li

Hanna Sanyour

Tyler Remund

Patrick Kelly

Zhongkui Hong

Affiliations

Soon will be listed here.

Abstract

Construction of an artificial vascular graft is widely considered a promising strategy in vascular tissue engineering. However, limited sources of functional vascular smooth muscle cells (VSMCs) remain a major obstacle in vascular tissue engineering. In this study, we innovatively developed an approach to obtain functional VSMCs by onsite differentiating human bone marrow-derived mesenchymal stem cells (MSCs) directed by decellularized extracellular matrix (ECM) and fibroblasts. The resulting cells and ECM-cells constructs were characterized by real time RT-PCR, immunofluorescence staining, cell contractile functions, and migration capacity. Our results showed both ECM and fibroblasts induced MSCs differentiation toward VSMC-like cells with increased transcription of marker genes, upregulated expression of contractile apparatus proteins, and enhanced functional activity of VSMC phenotype. Interestingly, our findings revealed that native ECM and fibroblasts-coculture had a higher potential to promote MSCs differentiation into VSMCs than growth factors cocktail (GFC) supplemented culture, thereby providing a potential source of VSMCs for blood vessel constitution.

Citing Articles

Research Progress of Fibroblasts in Human Diseases.

Li X, Li N, Wang Y, Han Q, Sun B Biomolecules. 2024; 14(11).

PMID: 39595654 PMC: 11591654. DOI: 10.3390/biom14111478.

The challenges and prospects of smooth muscle tissue engineering.

Baldwin C, Iyer S, Rao R Regen Med. 2024; 19(3):135-143.

PMID: 38440898 PMC: 10941056. DOI: 10.2217/rme-2023-0230.

Engineering Smooth Muscle to Understand Extracellular Matrix Remodeling and Vascular Disease.

Yarbrough D, Gerecht S Bioengineering (Basel). 2022; 9(9).

PMID: 36134994 PMC: 9495899. DOI: 10.3390/bioengineering9090449.

Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning.

Mayoral I, Bevilacqua E, Gomez G, Hmadcha A, Gonzalez-Loscertales I, Reina E Mater Today Bio. 2022; 14:100252.

PMID: 35509864 PMC: 9059085. DOI: 10.1016/j.mtbio.2022.100252.

Effects of Coculture Fibroblasts and Vascular Endothelial Cells on Proliferation and Osteogenesis of Adipose Stem Cells.

Liao X, Zhong R, Zhang H, Wang F Comput Math Methods Med. 2022; 2022:6288695.

PMID: 35069787 PMC: 8776444. DOI: 10.1155/2022/6288695.

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