Quantitation of Paracetamol by Liquid Chromatography-mass Spectrometry in Human Plasma in Support of Clinical Trial

Overview

Journal Future Sci OA

Specialty Biotechnology

Date 2018 Oct 2

PMID 30271617

Citations 5

Authors

Richard Kin-Ting Kam

Michael Ho-Ming Chan

Hiu-Ting Wong

Aniruddha Ghose

Arjen M Dondorp

Katherine Plewes

Joel Tarning

Affiliations

Soon will be listed here.

Abstract

Aim: Paracetamol is a well-tolerated antipyretic widely used in severe malaria management. The study aimed to develop and validate a rapid LC-MS/MS assay to quantify paracetamol in plasma from patients with severe malaria.

Materials & Methods: Plasma sample was precipitated by organic solvent containing isotope-labeled paracetamol internal standard. Supernatant was isolated, diluted with water, followed by LC-MS/MS analysis.

Results: Plasma samples were extracted and assayed in less than 5.5 min. The assay response was linear (0.125-50 mg/l) with total intra- and interassay imprecision of <1.4%, which were considerably lower than most published reports.

Conclusion: We developed, validated and applied a rapid and small volume LC-MS/MS assay with high precision and accuracy for plasma paracetamol quantitation in 989 samples from 62 patients with severe malaria. The simple and high-throughput quality could facilitate assay automation for future clinical studies.

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