GsMATE Encoding a Multidrug and Toxic Compound Extrusion Transporter Enhances Aluminum Tolerance in Arabidopsis Thaliana

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2018 Oct 1

PMID 30268093

Citations 22

Authors

Qibin Ma

Rong Yi

Lu Li

Zhongyi Liang

TingTing Zeng

Yu Zhang

He Huang

Xiao Zhang

Xiangli Yin

Zhandong Cai

Yinghui Mu

Yanbo Cheng

Qiaoying Zeng

Xiuping Li

Hai Nian

Affiliations

Soon will be listed here.

Abstract

Background: Multidrug and toxic compound extrusion (MATE) transporters, which exist widely in plants, function as crucial regulators in plant resistance to aluminum (Al) toxicity by inducing citrate efflux. However, the functions of most MATE family members in soybean (Glycine soja) remain to be elucidated.

Results: Expression pattern analysis showed that GsMATE was constitutively expressed in different soybean organs, with the highest level in root compared with those in stem, leaf and cotyledon. In addition, Al stress induced expression of GsMATE in soybean. Temporal analysis indicated that GsMATE expression was greatly enhanced by increasing concentrations of aluminum [Al] after short exposure, reaching the high levels detected in the BW69 (Al-resistant) and the JW81 (Al-sensitive) lines of Glycine soja of wild soybean at 6 h and 8 h, respectively. Furthermore, transient GsMATE expression in Arabidopsis protoplasts showed that GsMATE protein localized to the plasma membrane. Overexpression of GsMATE on an Arabidopsis columbia-0 (Col-0) background resulted in increased Al tolerance in transgenic plants. Analysis of hematoxylin staining showed that the roots of GsMATE transgenic lines were stained less intensely than those of the wild-type exposured to the same AlCl concentrations. Therefore, GsMATE enhanced the resistance of transgenic plants to Al toxicity by reducing Al accumulation in Arabidopsis roots.

Conclusions: In summary, our results indicate that GsMATE is responsive to aluminum stress and may participate in the regulation of sensitivity to Al toxicity in Arabidopsis. In addition, the GsMATE protein is an Al-induced citrate transporter of the MATE family and exerts an essential role in Al tolerance in Glycine soja.

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