High-affinity IgM Memory B Cells Are Defective in Differentiation into IgM Antibody-secreting Cells by Re-stimulation with a T Cell-dependent Antigen

Overview

Journal Sci Rep

Specialty Science

Date 2018 Sep 30

PMID 30266961

Citations 4

Authors

Yasuyuki Tashiro

Akikazu Murakami

Yasushi Hara

Takeyuki Shimizu

Masato Kubo

Ryo Goitsuka

Hidehiro Kishimoto

Takachika Azuma

Affiliations

Soon will be listed here.

Abstract

IgM antibodies (Abs) are thought to play a major role in humoral immunity but only at the early stage of the primary immune response. However, two subsets of IgM memory B cells (MBCs), one with high affinity gained by means of multiple somatic hypermutation (SHM) and the other with low affinity and no SHMs, are generated through the germinal center (GC)-dependent and GC-independent (non-GC) pathway, respectively, after immunization with (4-hydroxy-3-nitrophenyl)acetyl (NP)-chicken γ-globulin. Surprisingly, an analysis of antibody-secreting cells reveals that a large amount of anti-NP IgM Ab with few SHMs is secreted during the recall response, indicating that only non-GC MBCs have terminal differentiation potential. Since secondary IgM Abs are capable of binding to dinitrophenyl ligands, they likely provide broad cross-reactivity in defense against microbial infection.

Citing Articles

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