Chitosan and Chitooligosaccharides from Shrimp Shell Waste: Characterization, Antimicrobial and Shelf Life Extension in Bread

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2018 Sep 29

PMID 30263851

Citations 7

Authors

Numfon Rakkhumkaew

Chalinan Pengsuk

Affiliations

Soon will be listed here.

Abstract

Chitosan and chitooligosaccharides were extracted from white-leg shrimp shells by chemical treatment. Low molecular weight (13 kDa) and a high degree of deacetylation (54.83%) in chitooligosaccharides led to high water solubility compared to chitosan. Antimicrobial assays indicated that chitosan and chitooligosaccharides exhibited marked inhibitory activity against food-borne pathogenics, spoilage bacterial, and fungal strains tested. However, chitooligosaccharides revealed greater inhibitory effects than chitosan on tested microorganisms. The substitution of flour by chitosan or chitooligosaccharides in bread formulation (1 g/100 g total weight basis) showed antimicrobial effects against and sp. growth. Also, the fruity odor in bread containing chitosan or chitooligosaccharides was delayed. Interestingly, the bread containing chitooligosaccharides showed a stronger inhibitory effect against and sp. compared to bread containing chitosan and control, where and sp. were observed growing on the surface of bread after 4 days of incubation at 30 °C.

Citing Articles

Technological and microbiological characterization of an industrial soft-sliced bread enriched with chitosan and its prebiotic activity.

DAlessandro M, Schouten M, Gottardi D, Cortesi S, Romani S, Patrignani F Curr Res Food Sci. 2024; 9:100935.

PMID: 39697467 PMC: 11652935. DOI: 10.1016/j.crfs.2024.100935.

Chitooligosaccharide and Its Derivatives: Potential Candidates as Food Additives and Bioactive Components.

Mittal A, Singh A, Buatong J, Saetang J, Benjakul S Foods. 2023; 12(20).

PMID: 37893747 PMC: 10606384. DOI: 10.3390/foods12203854.

Bactericidal Action of Shrimp Shell Chitooligosaccharide Conjugated with Epigallocatechin Gallate (COS-EGCG) against .

Buatong J, Mittal A, Mittraparp-Arthorn P, Palamae S, Saetang J, Benjakul S Foods. 2023; 12(3).

PMID: 36766163 PMC: 9914238. DOI: 10.3390/foods12030634.

Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products.

Taokaew S, Kriangkrai W Biology (Basel). 2023; 12(1).

PMID: 36671779 PMC: 9855443. DOI: 10.3390/biology12010087.

Antioxidant Stress of Transdermal Gene Delivery by Non-Viral Gene Vectors Based on Chitosan-Oligosaccharide.

Cui P, Zhu T, Jiang P, Wang J J Funct Biomater. 2022; 13(4).

PMID: 36547559 PMC: 9781222. DOI: 10.3390/jfb13040299.

References

Ritthidej G, Phaechamud T, Koizumi T . Moist heat treatment on physicochemical change of chitosan salt films. Int J Pharm. 2002; 232(1-2):11-22. DOI: 10.1016/s0378-5173(01)00894-8. View

Di Martino A, Sittinger M, Risbud M . Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials. 2005; 26(30):5983-90. DOI: 10.1016/j.biomaterials.2005.03.016. View

Barboza-Corona J, Gutierrez-Acosta O, Imperial-Cervantes M, Bideshi D, de la Fuente-Salcido N, Bautista-Justo M . Generation of antibacterial oligosaccharides derived from chitin using heterologous endochitinase synthesized in Escherichia coli. J Appl Microbiol. 2009; 105(5):1511-20. DOI: 10.1111/j.1365-2672.2008.03904.x. View

Lafarga T, Gallagher E, Walsh D, Valverde J, Hayes M . Chitosan-containing bread made using marine shellfishery byproducts: functional, bioactive, and quality assessment of the end product. J Agric Food Chem. 2013; 61(37):8790-6. DOI: 10.1021/jf402248a. View

Helander I, Nurmiaho-Lassila E, Ahvenainen R, Rhoades J, Roller S . Chitosan disrupts the barrier properties of the outer membrane of gram-negative bacteria. Int J Food Microbiol. 2002; 71(2-3):235-44. DOI: 10.1016/s0168-1605(01)00609-2. View

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

Xiaoyan L, Yi L, Peng L, Songsheng Q, Ziniu Y . Microcalorimetric investigation on the growth model and the protein yield of Bacillus thuringiensis. J Biochem Biophys Methods. 2004; 59(3):267-74. DOI: 10.1016/j.jbbm.2004.03.003. View

No H, Meyers S, Prinyawiwatkul W, Xu Z . Applications of chitosan for improvement of quality and shelf life of foods: a review. J Food Sci. 2007; 72(5):R87-100. DOI: 10.1111/j.1750-3841.2007.00383.x. View

Sagoo S, Board R, Roller S . Chitosan potentiates the antimicrobial action of sodium benzoate on spoilage yeasts. Lett Appl Microbiol. 2002; 34(3):168-72. DOI: 10.1046/j.1472-765x.2002.01067.x. View

10.

No H, Park N, Lee S, Meyers S . Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol. 2002; 74(1-2):65-72. DOI: 10.1016/s0168-1605(01)00717-6. View

11.

Gerasimenko D, Avdienko I, Bannikova G, Zueva O, Varlamov V . [Antibacterial effects of water-soluble low-molecular-weight chitosans on different microorganisms]. Prikl Biokhim Mikrobiol. 2004; 40(3):301-6. View

12.

Lodhi G, Kim Y, Hwang J, Kim S, Jeon Y, Je J . Chitooligosaccharide and its derivatives: preparation and biological applications. Biomed Res Int. 2014; 2014:654913. PMC: 3958764. DOI: 10.1155/2014/654913. View

13.

Roller S, Covill N . The antifungal properties of chitosan in laboratory media and apple juice. Int J Food Microbiol. 1999; 47(1-2):67-77. DOI: 10.1016/s0168-1605(99)00006-9. View

14.

Baxter A, Dillon M, Taylor K, Roberts G . Improved method for i.r. determination of the degree of N-acetylation of chitosan. Int J Biol Macromol. 1992; 14(3):166-9. DOI: 10.1016/s0141-8130(05)80007-8. View