Characterization of Mannanase from Sp., a Novel Cell Wall-degrading Bacterium

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2018 Sep 29

PMID 30263731

Citations 8

Authors

Suae Kim

Mi-Hwa Lee

Eun-Sook Lee

Young-Do Nam

Dong-Ho Seo

Affiliations

Soon will be listed here.

Abstract

Seaweeds are considered as a health food partly due to the polysaccharide composition of the cell wall. Because conventional extraction methods have low yields and lead to environmental pollution, enzymatic methods have been proposed. In this study, a new strain of sp. was isolated from cattle feces that produced a mannanase, a polysaccharide-degrading enzyme active against the green seaweed . The purified 39-kDa mannanase exhibited maximum activity at 55 °C and pH 6.0, and maintained its catalytic activity stably at temperatures up to 60 °C and at a broad pH range (5.0-11.0). Enzymatic activity was slightly enhanced by Cu and Na but strongly inhibited by Fe, Ag, and EDTA. The mannanase showed the highest specificity to the inexpensive substrates such as konjac powder and locust bean gum, and efficiently released various manno-oligosaccharides. This novel mannanase can thus be applicable in the food, feed, and pulp industries.

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