Radioprotective Effect of Hesperetin Against γ-irradiation-induced DNA Damage and Immune Dysfunction in Murine Splenocytes

Overview

Journal Food Sci Biotechnol

Specialty Biotechnology

Date 2018 Sep 29

PMID 30263502

Citations 2

Authors

Jung Ae Kang

Seon Hye Yoon

Jong Kook Rho

Beom-Su Jang

Dae Seong Choi

Dong-Eun Lee

Eui-Baek Byun

Jongho Jeon

Sang Hyun Park

Affiliations

Soon will be listed here.

Abstract

This study was conducted to evaluate the preventive effect of hesperetin against radiation-induced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 µM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to ?-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.

Citing Articles

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Radioprotective Effect of Hesperidin: A Systematic Review.

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References

Mansour H, Hafez H, Fahmy N, Hanafi N . Protective effect of N-acetylcysteine against radiation induced DNA damage and hepatic toxicity in rats. Biochem Pharmacol. 2007; 75(3):773-80. DOI: 10.1016/j.bcp.2007.09.018. View

Trivedi P, Tripathi D, Jena G . Hesperetin protects testicular toxicity of doxorubicin in rat: role of NFκB, p38 and caspase-3. Food Chem Toxicol. 2010; 49(4):838-47. DOI: 10.1016/j.fct.2010.12.005. View

Rosenkranz A, Schmaldienst S, Stuhlmeier K, Chen W, Knapp W, Zlabinger G . A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin-diacetate. J Immunol Methods. 1992; 156(1):39-45. DOI: 10.1016/0022-1759(92)90008-h. View

C Jagetia G . Radioprotective Potential of Plants and Herbs against the Effects of Ionizing Radiation. J Clin Biochem Nutr. 2008; 40(2):74-81. PMC: 2127223. DOI: 10.3164/jcbn.40.74. View

Lee W, Ahn G, Lee B, Wijesinghe W, Kim D, Yang H . Radio-protective effect of polysaccharides isolated from Lactobacillus brevis-fermented Ecklonia cava. Int J Biol Macromol. 2012; 52:260-6. DOI: 10.1016/j.ijbiomac.2012.10.004. View

Jin Y, Han X, Zhang Y, Lee J, Lim Y, Kim T . Hesperetin, a bioflavonoid, inhibits rat aortic vascular smooth muscle cells proliferation by arresting cell cycle. J Cell Biochem. 2007; 104(1):1-14. DOI: 10.1002/jcb.21592. View

Szumiel I . Ionizing radiation-induced cell death. Int J Radiat Biol. 1994; 66(4):329-41. DOI: 10.1080/09553009414551271. View

Garg A, Garg S, Zaneveld L, Singla A . Chemistry and pharmacology of the Citrus bioflavonoid hesperidin. Phytother Res. 2001; 15(8):655-69. DOI: 10.1002/ptr.1074. View

Nair C, Parida D, Nomura T . Radioprotectors in radiotherapy. J Radiat Res. 2001; 42(1):21-37. DOI: 10.1269/jrr.42.21. View

10.

Kim J, Jung K, Choi J, Young Chung H . Hesperetin: a potent antioxidant against peroxynitrite. Free Radic Res. 2004; 38(7):761-9. DOI: 10.1080/10715760410001713844. View

11.

Smina T, De S, Devasagayam T, Adhikari S, Janardhanan K . Ganoderma lucidum total triterpenes prevent radiation-induced DNA damage and apoptosis in splenic lymphocytes in vitro. Mutat Res. 2011; 726(2):188-94. DOI: 10.1016/j.mrgentox.2011.09.005. View

12.

Hosoda M, Tokonami S, Tazoe H, Sorimachi A, Monzen S, Osanai M . Activity concentrations of environmental samples collected in Fukushima Prefecture immediately after the Fukushima nuclear accident. Sci Rep. 2013; 3:2283. PMC: 3724182. DOI: 10.1038/srep02283. View

13.

Ameer B, WEINTRAUB R, Johnson J, Yost R, Rouseff R . Flavanone absorption after naringin, hesperidin, and citrus administration. Clin Pharmacol Ther. 1996; 60(1):34-40. DOI: 10.1016/S0009-9236(96)90164-2. View

14.

Mates J . Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int J Biochem Cell Biol. 2000; 32(2):157-70. DOI: 10.1016/s1357-2725(99)00088-6. View

15.

Bing S, Kim M, Park E, Ahn G, Kim D, Ko R . 1,2,3,4,6-penta-O-galloyl-beta-D-glucose protects splenocytes against radiation-induced apoptosis in murine splenocytes. Biol Pharm Bull. 2010; 33(7):1122-7. DOI: 10.1248/bpb.33.1122. View

16.

Komarova E, Christov K, Faerman A, Gudkov A . Different impact of p53 and p21 on the radiation response of mouse tissues. Oncogene. 2000; 19(33):3791-8. DOI: 10.1038/sj.onc.1203717. View

17.

Hosoda M, Tokonami S, Sorimachi A, Monzen S, Osanai M, Yamada M . The time variation of dose rate artificially increased by the Fukushima nuclear crisis. Sci Rep. 2012; 1:87. PMC: 3216573. DOI: 10.1038/srep00087. View

18.

Lee N, Choi S, Park S, Park E, Kim D . Antiallergic activity of hesperidin is activated by intestinal microflora. Pharmacology. 2004; 71(4):174-80. DOI: 10.1159/000078083. View

19.

Ross G . Induction of cell death by radiotherapy. Endocr Relat Cancer. 2000; 6(1):41-4. DOI: 10.1677/erc.0.0060041. View

20.

Zhou D, Yu T, Chen G, Brown S, Yu Z, Mattson M . Effects of NF-kappaB1 (p50) targeted gene disruption on ionizing radiation-induced NF-kappaB activation and TNFalpha, IL-1alpha, IL-1beta and IL-6 mRNA expression in vivo. Int J Radiat Biol. 2001; 77(7):763-72. DOI: 10.1080/09553000110050047. View