Species-dependent Isoenzyme Subtypes of Membrane-bound Cyclic AMP-dependent Protein Kinase in Highly Purified Cardiac Sarcolemma

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Sep 1

PMID 3026349

Authors

J G CHURCH

J B Derdemezi

S Yuan

A K Sen

Affiliations

Soon will be listed here.

Abstract

Highly purified sarcolemma from dog and pig cardiac muscle has been shown to contain significant activities of a membrane-bound cyclic AMP-dependent protein kinase. In addition, these membranes undergo endogenous phosphorylation when incubated with Mg2+ and [gamma-32P]ATP. By comparing 32P-labelled patterns obtained with [gamma-32P]ATP and the photoaffinity label 8-azidoadenosine 3':5'-[32P]monophosphate (8-azido-cyclic [32P]AMP), we have demonstrated that, whereas the major kinase isoenzyme in dog sarcolemma was Type II, that in the pig membrane was the Type I isoenzyme.

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