Aromatic Hydroxylation As a Potential Measure of Hydroxyl-radical Formation in Vivo. Identification of Hydroxylated Derivatives of Salicylate in Human Body Fluids

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Jul 15

PMID 3026319

Citations 47

Authors

M Grootveld

B Halliwell

Affiliations

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Abstract

Attack by .OH radicals, generated by a Fenton system, upon salicylate produces 2,3-dihydroxybenzoate and 2,5-dihydroxybenzoate as major products and catechol as a minor product. H.p.l.c. separation combined with electrochemical detection was used to identify and quantify 2,3-dihydroxybenzoate and 2,5-dihydroxybenzoate in human plasma and synovial fluid. We propose that conversion of salicylate into 2,3-dihydroxybenzoate, or of other aromatic compounds into specific hydroxylated products, may be a useful assay for .OH formation in the human body.

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