Sex Differences in Forkhead Box O3a Signaling Response to Hindlimb Unloading in Rat Soleus Muscle

Overview

Journal J Physiol Sci

Specialty Physiology

Date 2018 Sep 28

PMID 30259391

Citations 16

Authors

Toshinori Yoshihara

Toshiharu Natsume

Takamasa Tsuzuki

Shuo-Wen Chang

Ryo Kakigi

Takao Sugiura

Hisashi Naito

Affiliations

Soon will be listed here.

Abstract

We tested the hypothesis that there are sex differences in hindlimb unloading-induced activation of the forkhead box subfamily O3a (FoxO3a) signaling pathway in rat soleus muscle. Age-matched male and female Wistar rats were subjected to hindlimb unloading, and the soleus muscle was removed before or 1 or 7 days after unloading. Female rats showed greater percent changes in relative soleus muscle weight than males. FoxO3a phosphorylation was lower in females than in males and was associated with higher levels of protein ubiquitination 7 days after unloading. Heat shock protein 72 (Hsp72) levels were lower in female rats and increased in males during unloading. Female rats showed slightly higher myostatin levels, which showed a non-significant decline in male rats following unloading. Thus, males and females show different responses to the FoxO3a/ubiquitin-proteasome pathway following hindlimb unloading in rat soleus muscle, which may be associated with differences in Hsp72 expression and myostatin signaling.

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