Redox Balance and DNA Fragmentation in Arsenic-exposed Occupational Workers from Different Industries of Pakistan

Overview

Journal Environ Sci Pollut Res Int

Publisher Springer

Specialties Environmental Health
Toxicology

Date 2018 Sep 28

PMID 30259327

Citations 2

Authors

Maryam Raza

Ishrat Mahjabeen

Muhammad Fahim

Waqar Ahmad Malik

Asad Ullah Khan

Mahmood Akhtar Kayani

Ayesha Khan

Zertashia Akram

Affiliations

Soon will be listed here.

Abstract

AbstractOccupational exposure accounts for a contact between workers and different toxicants. Present study was designed to measure the arsenic-induced DNA fragmentation and oxidative stress in exposed workers. Blood, hair, and nail samples were collected from welding, brick kiln, furniture, pesticide, and paint industries (n = 50/industry) of Pakistan along with 200 controls. DNA damage was calculated using DNA fragmentation assay. Antioxidant enzymes (CAT, SOD, GPx) were measured using ELISA. Results revealed that arsenic exposure induced DNA fragmentation in brick kiln, furniture, and welding industries. Enzyme activity was reduced in five industries compared to control. In exposed group, significant depletion of enzymes was observed in furniture, welding, and brick kiln workers. Based on age and time of exposure, significant difference was observed in welding and brick kiln group. Smokers of exposed group showed significantly reduced levels of enzymes compared to controls. Arsenic deposition was observed higher in the hair, nail, and blood samples of exposed group (P < 0.001) compared to control. Likewise, lead and cadmium contents were higher in the blood samples of industrial workers compared to control. This study suggests increased trend of cellular damage and oxidative stress in occupational workers profoundly in welding, furniture, and brick kiln industries. Moreover, this study recognizes the contribution of age, exposure time, and smoking status toward arsenic-induced oxidative stress and DNA fragmentation.

Citing Articles

Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers.

Akram Z, Mahjabeen I, Umair M, Fahim M, Kayani M, Fatima L PLoS One. 2022; 17(9):e0273211.

PMID: 36178927 PMC: 9524634. DOI: 10.1371/journal.pone.0273211.

Association of intronic polymorphisms (rs1549339, rs13402242) and mRNA expression variations in PSMD1 gene in arsenic-exposed workers.

Ahmad S, Arif B, Akram Z, Ahmed M, Khan A, Hussain M Environ Sci Pollut Res Int. 2020; 27(10):11425-11437.

PMID: 31965495 DOI: 10.1007/s11356-019-07422-x.

References

Halatek T, Sinczuk-Walczak H, Rabieh S, Wasowicz W . Association between occupational exposure to arsenic and neurological, respiratory and renal effects. Toxicol Appl Pharmacol. 2009; 239(2):193-9. DOI: 10.1016/j.taap.2009.04.022. View

Abernathy C, Liu Y, LONGFELLOW D, APOSHIAN H, Beck B, Fowler B . Arsenic: health effects, mechanisms of actions, and research issues. Environ Health Perspect. 1999; 107(7):593-7. PMC: 1566656. DOI: 10.1289/ehp.99107593. View

Gobba N, Hussein Ali A, El Sharawy D, Hussein M . The potential hazardous effect of exposure to iron dust in Egyptian smoking and nonsmoking welders. Arch Environ Occup Health. 2017; 73(3):189-202. DOI: 10.1080/19338244.2017.1314930. View

Manna P, Sinha M, Sil P . Arsenic-induced oxidative myocardial injury: protective role of arjunolic acid. Arch Toxicol. 2008; 82(3):137-49. DOI: 10.1007/s00204-007-0272-8. View

Valko M, Morris H, Cronin M . Metals, toxicity and oxidative stress. Curr Med Chem. 2005; 12(10):1161-208. DOI: 10.2174/0929867053764635. View

Muniz J, McCauley L, Scherer J, Lasarev M, Koshy M, Kow Y . Biomarkers of oxidative stress and DNA damage in agricultural workers: a pilot study. Toxicol Appl Pharmacol. 2007; 227(1):97-107. DOI: 10.1016/j.taap.2007.10.027. View

Rubino F . Toxicity of Glutathione-Binding Metals: A Review of Targets and Mechanisms. Toxics. 2017; 3(1):20-62. PMC: 5634692. DOI: 10.3390/toxics3010020. View

Hughes M . Arsenic toxicity and potential mechanisms of action. Toxicol Lett. 2002; 133(1):1-16. DOI: 10.1016/s0378-4274(02)00084-x. View

Bibi M, Hashmi M, Malik R . Human exposure to arsenic in groundwater from Lahore district, Pakistan. Environ Toxicol Pharmacol. 2014; 39(1):42-52. DOI: 10.1016/j.etap.2014.10.020. View

10.

Ko J, Cheng Y, Liu G, Hsin I, Chen H . The association of occupational metals exposure and oxidative damage, telomere shortening in fitness equipments manufacturing workers. Ind Health. 2017; 55(4):345-353. PMC: 5546843. DOI: 10.2486/indhealth.2016-0148. View

11.

Hong Y, Song K, Chung J . Health effects of chronic arsenic exposure. J Prev Med Public Health. 2014; 47(5):245-52. PMC: 4186552. DOI: 10.3961/jpmph.14.035. View

12.

Barata C, Varo I, Navarro J, Arun S, Porte C . Antioxidant enzyme activities and lipid peroxidation in the freshwater cladoceran Daphnia magna exposed to redox cycling compounds. Comp Biochem Physiol C Toxicol Pharmacol. 2005; 140(2):175-86. DOI: 10.1016/j.cca.2005.01.013. View

13.

Cassini C, Calloni C, Bortolini G, Garcia S, Dornelles M, Henriques J . Occupational risk assessment of oxidative stress and genotoxicity in workers exposed to paints during a working week. Int J Occup Med Environ Health. 2011; 24(3):308-19. DOI: 10.2478/s13382-011-0030-2. View

14.

Vahter M, Concha G . Role of metabolism in arsenic toxicity. Pharmacol Toxicol. 2001; 89(1):1-5. DOI: 10.1034/j.1600-0773.2001.d01-128.x. View

15.

Petrick J, Cullen W, Carter D, Aposhian H . Monomethylarsonous acid (MMA(III)) is more toxic than arsenite in Chang human hepatocytes. Toxicol Appl Pharmacol. 2000; 163(2):203-7. DOI: 10.1006/taap.1999.8872. View

16.

Liu H, Lin M, Liu P, Chan C, Chen H . Health risk assessment by measuring plasma malondialdehyde (MDA), urinary 8-hydroxydeoxyguanosine (8-OH-dG) and DNA strand breakage following metal exposure in foundry workers. J Hazard Mater. 2009; 170(2-3):699-704. DOI: 10.1016/j.jhazmat.2009.05.010. View

17.

Ishaq M, Khan M, Akbar Jan F, Ahmad I . Heavy metals in brick kiln located area using atomic absorption spectrophotometer: a case study from the city of Peshawar, Pakistan. Environ Monit Assess. 2009; 166(1-4):409-20. DOI: 10.1007/s10661-009-1011-9. View

18.

Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D . Arsenic: toxicity, oxidative stress and human disease. J Appl Toxicol. 2011; 31(2):95-107. DOI: 10.1002/jat.1649. View

19.

Styblo M, Del Razo L, Vega L, Germolec D, LeCluyse E, Hamilton G . Comparative toxicity of trivalent and pentavalent inorganic and methylated arsenicals in rat and human cells. Arch Toxicol. 2000; 74(6):289-99. DOI: 10.1007/s002040000134. View

20.

Tchounwou P, Patlolla A, Centeno J . Carcinogenic and systemic health effects associated with arsenic exposure--a critical review. Toxicol Pathol. 2003; 31(6):575-88. DOI: 10.1080/01926230390242007. View