Nucleolus Vs Nucleus Count for Identifying Spiral Ganglion in Human Temporal Bone

Overview

Journal J Int Adv Otol

Publisher Aves

Specialty Otorhinolaryngology

Date 2018 Sep 27

PMID 30256195

Citations 2

Authors

Arianna Di Stadio

Massimo Ralli

Reuven Ishai

Luca DAscanio

Franco Trabalzini

Antonio Della Volpe

Gregorio Babighian

Giampietro Ricci

Affiliations

Soon will be listed here.

Abstract

Objectives: Spiral ganglion (SG) counting is used in experimental studies conducted on age-, noise-, and drug-induced sensorineural hearing loss, as well as in the assessment of cochlear implant performances. Different methods of counting have been reported, but no definite standardization of such procedure has been published. The aim of our study is to identify the best method to count human spiral ganglions (SGs).

Materials And Methods: By identification of nuclei or nucleoli as described by Schucknect, seven researchers with different experience levels counted SGs in 123 human temporal bones (TBs). Data on time of post-mortem bone removal post-mortem, methods of specimen's fixation, decalcification, and coloration were collected to test their possible influence on human tissue. Percentage, two-tailed t-test, Spearman's test, and one-way ANOVA were used to analyze the data.

Results: Nucleoli were identified in 61% of cases, whereas nuclei were recognized in 100% of cases (p<0.005). Nucleoli presence in all four segments in the same temporal bone (TB) was observed in 69 cases (92%), whereas nuclei were identified in all four segments in 103 cases (83.7%) (p<0.001). The junior investigators requested a double check by the seniors in 25 (20.3%) cases for identifying and counting nucleoli, whereas the senior researchers showed no doubts in their identification and count. The only parameter positively affecting nucleoli identification in tissue preparation was bone removal for <12 h with respect to longer post-mortem time (p<0.001).

Conclusion: We suggest counting nuclei, rather than nucleoli, for spiral ganglion computation because of easier recognition of nuclei, especially in case of investigator's limited experience.

Citing Articles

Spiral Ganglions and Speech Perception in the Elderly. Which Turn of the Cochlea is the More Relevant? A Preliminary Study on Human Temporal Bones.

Stadio A, Volpe A, Ralli M, Korsch F, Greco A, Ricci G J Int Adv Otol. 2020; 16(3):318-322.

PMID: 33136010 PMC: 7901449. DOI: 10.5152/iao.2020.8481.

E-ABR in Patients with Cochlear Implant: A Comparison between Patients with Malformed Cochlea and Normal Cochlea.

Stadio A, Dipietro L, De Lucia A, Trabalzini F, Ricci G, Martines F J Int Adv Otol. 2019; 15(2):215-221.

PMID: 31418713 PMC: 6750786. DOI: 10.5152/iao.2019.6251.

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