Comparing Arabidopsis Receptor Kinase and Receptor Protein-mediated Immune Signaling Reveals BIK1-dependent Differences

Overview

Journal New Phytol

Specialty Biology

Date 2018 Sep 26

PMID 30252144

Citations 27

Authors

Wei-Lin Wan

Lisha Zhang

Rory Pruitt

Maricris Zaidem

Rik Brugman

Xiyu Ma

Elzbieta Krol

Artemis Perraki

Joachim Kilian

Guido Grossmann

Mark Stahl

Libo Shan

Cyril Zipfel

Jan A L van Kan

Rainer Hedrich

Detlef Weigel

Andrea A Gust

Thorsten Nurnberger

Affiliations

Soon will be listed here.

Abstract

Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine-rich repeat receptor kinases (LRR-RK) FLS2 and EFR, and the LRR receptor protein (LRR-RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR-RK and LRR-RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP-mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23-regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2-signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP-type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.

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