Accurate in Vitro Transcription of Xenopus Laevis Mitochondrial DNA from Two Bidirectional Promoters

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1986 Jul 1

PMID 3023938

Citations 21

Authors

D F Bogenhagen

B K Yoza

Affiliations

Soon will be listed here.

Abstract

The mitochondrial RNA polymerase from Xenopus laevis oocytes was partially purified by heparin-Sepharose chromatography and phosphocellulose chromatography. This RNA polymerase preparation specifically initiated the transcription of X. laevis mitochondrial DNA (mtDNA) from two bidirectional promoters contained within a 123-base-pair segment of the mtDNA between the heavy-strand replication origin and the rRNA cistrons. Transcription in vitro initiated from precisely the same start sites previously mapped as initiation sites for transcription in vivo. At each of the four sites, initiation occurred within a conserved nucleotide sequence, ACPuTTATA. This consensus sequence is not related to promoters for transcription of human mtDNA.

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