Chloroplast Protein 12 Expression Alters Growth and Chilling Tolerance in Tropical Forage (Aublet) Sw

Overview

Journal Front Plant Sci

Date 2018 Sep 22

PMID 30237807

Citations 8

Authors

Kailong Li

Hong Qiu

Min Zhou

Yang Lin

Zhenfei Guo

Shaoyun Lu

Affiliations

Soon will be listed here.

Abstract

(Aublet) Sw. is a tropical forage legume with soil acidity tolerance and excellent adaptation to infertile soils, but sensitive to chilling. To understand the molecular responses of to chilling, differentially expressed genes between a chilling tolerant mutant 7-1 and the wild type were identified using suppression subtractive hybridization, and eight of them were confirmed and the regulation pattern were analyzed using quantitative reverse transcription PCR (qRT-PCR). Chloroplast protein 12 (CP12) functions to regulate the Calvin cycle by forming a ternary complex with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK). transcript was induced by chilling in both plants, and higher levels were observed in 7-1 than in the wild type, implying a potential role of in chilling tolerance. To confirm this, transgenic plants over-expressing or down-regulating were generated, respectively. Higher / and survival rate and lower ion leakage were observed in transgenic plants overexpressing as compared with the wild type after chilling treatment, while lower / and survival rate and higher ion leakage were found in antisense plants. overexpression plants showed promoted growth with increased plant height and fresh weight, while the antisense plants exhibited reduced growth with decreased plant height and fresh weight as compared with the wild type. The results indicated that regulation of expression alters plant growth and chilling tolerance in . In addition, higher levels of net photosynthetic rate (), GAPDH and PRK activities were observed in overexpression transgenic plants, while lower levels in antisense plants than in the wild type under both control and chilling conditions, indicating that altered activities of GAPDH and PRK were associated with the changed in transgenic . Our results suggest that SgCP12 regulates GAPDH and PRK activities, , and chilling tolerance in .

Citing Articles

NTRC regulates CP12 to activate Calvin-Benson cycle during cold acclimation.

Teh J, Leitz V, Holzer V, Neusius D, Marino G, Meitzel T Proc Natl Acad Sci U S A. 2023; 120(33):e2306338120.

PMID: 37549282 PMC: 10433458. DOI: 10.1073/pnas.2306338120.

Conformational Disorder Analysis of the Conditionally Disordered Protein CP12 from in Its Different Redox States.

Del Giudice A, Gurrieri L, Galantini L, Fanti S, Trost P, Sparla F Int J Mol Sci. 2023; 24(11).

PMID: 37298260 PMC: 10253348. DOI: 10.3390/ijms24119308.

A Trajectory of Discovery: Metabolic Regulation by the Conditionally Disordered Chloroplast Protein, CP12.

Gerard C, Carriere F, Receveur-Brechot V, Launay H, Gontero B Biomolecules. 2022; 12(8).

PMID: 36008940 PMC: 9406205. DOI: 10.3390/biom12081047.

Reduction in Phosphoribulokinase Amount and Re-Routing Metabolism in CP12 Mutants.

Gerard C, Lebrun R, Lemesle E, Avilan L, Chang K, Jin E Int J Mol Sci. 2022; 23(5).

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Flexibility of Oxidized and Reduced States of the Chloroplast Regulatory Protein CP12 in Isolation and in Cell Extracts.

Launay H, Shao H, Bornet O, Cantrelle F, Lebrun R, Receveur-Brechot V Biomolecules. 2021; 11(5).

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