Molecular and Physiological Study of by Quantitative Proteome Analysis

Overview

Journal Proteomes

Date 2018 Sep 21

PMID 30231513

Citations 3

Authors

Seiji Shibasaki

Miki Karasaki

Wataru Aoki

Mitsuyoshi Ueda

Affiliations

Soon will be listed here.

Abstract

is one of the major pathogens that cause the serious infectious condition known as candidiasis. was investigated by proteome analysis to systematically examine its virulence factors and to promote the development of novel pharmaceuticals against candidiasis. Here, we review quantitative time-course proteomics data related to adaptation to fetal bovine serum, which were obtained using a nano-liquid chromatography/tandem mass spectrometry system equipped with a long monolithic silica capillary column. It was revealed that induced proteins involved in iron acquisition, detoxification of oxidative species, energy production, and pleiotropic stress tolerance. Native interactions of with macrophages were also investigated with the same proteome-analysis system. Simultaneous analysis of and macrophages without isolating individual living cells revealed an attractive strategy for studying the survival of . Although those data were obtained by performing proteome analyses, the molecular physiology of is discussed and trials related to pharmaceutical applications are also examined.

Citing Articles

Unveiling the roles of in : Implications for virulence and azole resistance.

Huang M, Song D, Zhou L, Jiao Z, Yang L, Yang Y Virulence. 2024; 15(1):2405000.

PMID: 39403939 PMC: 11485852. DOI: 10.1080/21505594.2024.2405000.

Promising immunotherapeutic targets for treating candidiasis.

Feng Z, Lu H, Jiang Y Front Cell Infect Microbiol. 2024; 14:1339501.

PMID: 38404288 PMC: 10884116. DOI: 10.3389/fcimb.2024.1339501.

The Role of B-Cells and Antibodies against Vaccine Antigens in Invasive Candidiasis.

Shukla M, Chandley P, Rohatgi S Vaccines (Basel). 2021; 9(10).

PMID: 34696267 PMC: 8540628. DOI: 10.3390/vaccines9101159.

References

Motokawa M, Kobayashi H, Ishizuka N, Minakuchi H, Nakanishi K, Jinnai H . Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography. J Chromatogr A. 2002; 961(1):53-63. DOI: 10.1016/s0021-9673(02)00133-4. View

Becker J, Kauffman S, Hauser M, Huang L, Lin M, Sillaots S . Pathway analysis of Candida albicans survival and virulence determinants in a murine infection model. Proc Natl Acad Sci U S A. 2010; 107(51):22044-9. PMC: 3009777. DOI: 10.1073/pnas.1009845107. View

Aoki W, Kitahara N, Miura N, Morisaka H, Kuroda K, Ueda M . Design of a novel antimicrobial peptide activated by virulent proteases. Chem Biol Drug Des. 2012; 80(5):725-33. DOI: 10.1111/cbdd.12012. View

Martin-Manso G, Navarathna D, Galli S, Soto-Pantoja D, Kuznetsova S, Tsokos M . Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis. PLoS One. 2012; 7(11):e48775. PMC: 3492437. DOI: 10.1371/journal.pone.0048775. View

Gasparoto T, Gaziri L, Burger E, Almeida R, Felipe I . Apoptosis of phagocytic cells induced by Candida albicans and production of IL-10. FEMS Immunol Med Microbiol. 2004; 42(2):219-24. DOI: 10.1016/j.femsim.2004.05.006. View

Edfors F, Danielsson F, Hallstrom B, Kall L, Lundberg E, Ponten F . Gene-specific correlation of RNA and protein levels in human cells and tissues. Mol Syst Biol. 2016; 12(10):883. PMC: 5081484. DOI: 10.15252/msb.20167144. View

Aoki W, Tatsukami Y, Kitahara N, Matsui K, Morisaka H, Kuroda K . Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics. J Proteomics. 2013; 91:417-29. DOI: 10.1016/j.jprot.2013.07.031. View

Axsen W, Styer C, Solnick J . Inhibition of heat shock protein expression by Helicobacter pylori. Microb Pathog. 2009; 47(4):231-6. PMC: 5494282. DOI: 10.1016/j.micpath.2009.08.002. View

Aoki W, Kitahara N, Miura N, Morisaka H, Yamamoto Y, Kuroda K . Comprehensive characterization of secreted aspartic proteases encoded by a virulence gene family in Candida albicans. J Biochem. 2011; 150(4):431-8. DOI: 10.1093/jb/mvr073. View

10.

Yamada-Okabe T, Yamada-Okabe H . Characterization of the CaNAG3, CaNAG4, and CaNAG6 genes of the pathogenic fungus Candida albicans: possible involvement of these genes in the susceptibilities of cytotoxic agents. FEMS Microbiol Lett. 2002; 212(1):15-21. DOI: 10.1111/j.1574-6968.2002.tb11238.x. View

11.

Staniszewska M, Bondaryk M, Malewski T, Kurzatkowski W . Quantitative expression of Candida albicans aspartyl proteinase genes SAP7, SAP8, SAP9, SAP10 in human serum in vitro. Pol J Microbiol. 2014; 63(1):15-20. View

12.

de Hoon M, Imoto S, Nolan J, Miyano S . Open source clustering software. Bioinformatics. 2004; 20(9):1453-4. DOI: 10.1093/bioinformatics/bth078. View

13.

Phan Q, Myers C, Fu Y, Sheppard D, Yeaman M, Welch W . Als3 is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells. PLoS Biol. 2007; 5(3):e64. PMC: 1802757. DOI: 10.1371/journal.pbio.0050064. View

14.

Xu X, Lee R, Fang H, Wang Y, Li R, Zou H . Bacterial peptidoglycan triggers Candida albicans hyphal growth by directly activating the adenylyl cyclase Cyr1p. Cell Host Microbe. 2008; 4(1):28-39. DOI: 10.1016/j.chom.2008.05.014. View

15.

Sasi B, Sonawane P, Gupta V, Sahu B, Mahapatra N . Coordinated transcriptional regulation of Hspa1a gene by multiple transcription factors: crucial roles for HSF-1, NF-Y, NF-κB, and CREB. J Mol Biol. 2013; 426(1):116-35. DOI: 10.1016/j.jmb.2013.09.008. View

16.

De Bernardis F, Muhlschlegel F, Cassone A, Fonzi W . The pH of the host niche controls gene expression in and virulence of Candida albicans. Infect Immun. 1998; 66(7):3317-25. PMC: 108348. DOI: 10.1128/IAI.66.7.3317-3325.1998. View

17.

Desai P, Thakur A, Ganguli D, Paul S, Morschhauser J, Bachhawat A . Glutathione utilization by Candida albicans requires a functional glutathione degradation (DUG) pathway and OPT7, an unusual member of the oligopeptide transporter family. J Biol Chem. 2011; 286(48):41183-41194. PMC: 3308832. DOI: 10.1074/jbc.M111.272377. View

18.

Tylicki A, Ziolkowska G, Bolkun A, Siemieniuk M, Czerniecki J, Nowakiewicz A . Comparative study of the activity and kinetic properties of malate dehydrogenase and pyruvate decarboxylase from Candida albicans, Malassezia pachydermatis, and Saccharomyces cerevisiae. Can J Microbiol. 2008; 54(9):734-41. DOI: 10.1139/w08-062. View

19.

Ibata-Ombetta S, Idziorek T, Trinel P, Poulain D, Jouault T . Candida albicans phospholipomannan promotes survival of phagocytosed yeasts through modulation of bad phosphorylation and macrophage apoptosis. J Biol Chem. 2003; 278(15):13086-93. DOI: 10.1074/jbc.M210680200. View

20.

Buu L, Chen Y . Sap6, a secreted aspartyl proteinase, participates in maintenance the cell surface integrity of Candida albicans. J Biomed Sci. 2014; 20:101. PMC: 3890532. DOI: 10.1186/1423-0127-20-101. View