Prunetin Relaxed Isolated Rat Aortic Rings by Blocking Calcium Channels

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2018 Sep 20

PMID 30227625

Citations 7

Authors

Bumjung Kim

Cheolmin Jo

Ho-Young Choi

Kyungjin Lee

Affiliations

Soon will be listed here.

Abstract

Prunetin, a component of herbal medicines and various foods, such as pea, peach, cherry, and , is a useful pharmacological compound. We previously reported the potent vasorelaxant effect of the bark of . Therefore, we investigated the vasorelaxant activities of prunetin on isolated rat aortic rings and hypotensive activity on spontaneously hypertensive rats (SHR) in this study. In the present study, prunetin (1⁻30 μg/mL) relaxed isolated rat aortic rings pre-contracted by phenylephrine (PE) in a concentration-dependent manner. Pre-incubation with prunetin (3 and 10 μg/mL) inhibited vasoconstriction induced by the supply of Ca in rat aortic rings pre-contracted with PE or KCl in a Ca-free Krebs⁻Henseleit (KH) buffer. Prunetin (10 μg/mL) pre-treatment also inhibited caffeine-induced contraction of aortic rings in a Ca-free KH buffer. To investigate the hypotensive effect of prunetin, the systolic blood pressure (SBP) of the SHR was measured by using a tail cuff assay. The SBP of SHR was significantly lower in the prunetin (25 mg/kg)-treated group. These results suggested that prunetin decreased blood pressure and relaxed blood vessels by blocking receptor-operated calcium channels, voltage-dependent calcium channels, and ryanodine receptor channels.

Citing Articles

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