Tetrahydropalmatine Prevents High-Fat Diet-Induced Hyperlipidemia in Golden Hamsters (Mesocricetus Auratus)

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2018 Sep 19

PMID 30226834

Citations 6

Authors

Caihua Sun

Zhiyun Chen

Hui Wang

Ke Ding

Affiliations

Soon will be listed here.

Abstract

BACKGROUND Hyperlipidemia is a major cause of atherosclerotic cardiovascular disease. Tetrahydropalmatine (THP) can exhibit hepatoprotective, anti-arrhythmic, and anti-inflammatory activities. The mechanism of THP on the hyperlipidemia remains unknown; therefore, the present study explored the role of THP in hyperlipidemia. MATERIAL AND METHODS We established an animal model of hyperlipidemia by high-fat diet (HFD) feeding. Blood samples were obtained for determination of serum cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), pro-inflammatory cytokines, and CYP7A1 expression. Histology was performed and inflammation was detected in the liver using hematoxylin-eosin (HE) staining and enzyme-linked immunosorbent assay (ELISA), respectively. The mRNA and protein levels of TLR4 and TRAF-6 were determined by quantitative real-time PCR (qPCR) and Western blot, respectively. RESULTS THP suppressed hepatic lipid accumulation and reduced serum levels of TC, TG, LDL-c, and HDL-c in HFD-fed golden hamsters. THP increased cholesterol 7 a-hydroxylase (CYP7A1) expression and prevented inflammation by the limited reduction in interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expressions in serum and liver. THP slightly increased the ratio of the body/liver weight. THP inhibited the mRNA and protein levels of Toll-like receptor 4 (TLR4) and TNF-receptor associated factor-6 (TRAF-6). CONCLUSIONS These results suggest that THP attenuates hyperlipidemia by multiple effects, including hepatoprotective and anti-inflammatory effects. Moreover, THP also suppressed the expressions of TLR4 and TRAF-6 in golden hamsters.

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References

Seok S, Park T, Park H, Baik E, Lee S . Fructose-1,6-bisphosphate suppresses lipopolysaccharide-induced expression of ICAM-1 through modulation of toll-like receptor-4 signaling in brain endothelial cells. Int Immunopharmacol. 2015; 26(1):203-11. DOI: 10.1016/j.intimp.2015.03.029. View

Nishimura S, Manabe I, Nagasaki M, Eto K, Yamashita H, Ohsugi M . CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity. Nat Med. 2009; 15(8):914-20. DOI: 10.1038/nm.1964. View

Cao X, Du J, Zhang Y, Yan J, Hu X . Hyperlipidemia exacerbates cerebral injury through oxidative stress, inflammation and neuronal apoptosis in MCAO/reperfusion rats. Exp Brain Res. 2015; 233(10):2753-65. DOI: 10.1007/s00221-015-4269-x. View

Lai L, Chen Y, Tian X, Li X, Zhang X, Lei J . Artesunate alleviates hepatic fibrosis induced by multiple pathogenic factors and inflammation through the inhibition of LPS/TLR4/NF-κB signaling pathway in rats. Eur J Pharmacol. 2015; 765:234-41. DOI: 10.1016/j.ejphar.2015.08.040. View

Zhao N, Chen Y, Zhu J, Wang L, Cao G, Dang Y . Levo-tetrahydropalmatine attenuates the development and expression of methamphetamine-induced locomotor sensitization and the accompanying activation of ERK in the nucleus accumbens and caudate putamen in mice. Neuroscience. 2013; 258:101-10. DOI: 10.1016/j.neuroscience.2013.11.025. View

Wang W, He Y, Lin P, Li Y, Sun R, Gu W . In vitro effects of active components of Polygonum Multiflorum Radix on enzymes involved in the lipid metabolism. J Ethnopharmacol. 2014; 153(3):763-70. DOI: 10.1016/j.jep.2014.03.042. View

Yang Y, Wu T, He K, Fu Z . Effect of aerobic exercise and ginsenosides on lipid metabolism in diet-induced hyperlipidemia mice. Zhongguo Yao Li Xue Bao. 2000; 20(6):563-5. View

Chow E, Magomedova L, Quach H, Patel R, Durk M, Fan J . Vitamin D receptor activation down-regulates the small heterodimer partner and increases CYP7A1 to lower cholesterol. Gastroenterology. 2013; 146(4):1048-59. DOI: 10.1053/j.gastro.2013.12.027. View

Liu J, Zhuang Z, Bian D, Ma X, Xun Y, Yang W . Toll-like receptor-4 signalling in the progression of non-alcoholic fatty liver disease induced by high-fat and high-fructose diet in mice. Clin Exp Pharmacol Physiol. 2014; 41(7):482-8. DOI: 10.1111/1440-1681.12241. View

10.

Lucas E, Lightfoot S, Hammond L, Devareddy L, Khalil D, Daggy B . Soy isoflavones prevent ovariectomy-induced atherosclerotic lesions in Golden Syrian hamster model of postmenopausal hyperlipidemia. Menopause. 2003; 10(4):314-21. DOI: 10.1097/01.GME.0000051509.84118.FD. View

11.

Alsheikh-Ali A, Kuvin J, Karas R . Risk of adverse events with fibrates. Am J Cardiol. 2004; 94(7):935-8. DOI: 10.1016/j.amjcard.2004.06.033. View

12.

Wang S, Zhang X, Zhai L, Sheng X, Zheng W, Chu H . Atorvastatin Attenuates Cognitive Deficits and Neuroinflammation Induced by Aβ Involving Modulation of TLR4/TRAF6/NF-κB Pathway. J Mol Neurosci. 2018; 64(3):363-373. DOI: 10.1007/s12031-018-1032-3. View

13.

McGrath K, Li X, Whitworth P, Kasz R, Tan J, McLennan S . High density lipoproteins improve insulin sensitivity in high-fat diet-fed mice by suppressing hepatic inflammation. J Lipid Res. 2013; 55(3):421-30. PMC: 3934727. DOI: 10.1194/jlr.M043281. View

14.

Jain K, Kathiravan M, Somani R, Shishoo C . The biology and chemistry of hyperlipidemia. Bioorg Med Chem. 2007; 15(14):4674-99. DOI: 10.1016/j.bmc.2007.04.031. View

15.

Yu J, Che J, Liu L, Yang F, Zhu X, Cao B . Tetrahydropalmatine attenuates irradiation induced lung injuries in rats. Life Sci. 2016; 153:74-81. DOI: 10.1016/j.lfs.2016.03.056. View

16.

Cao G, Zhang Y, Zhu L, Zhu J, Zhao N, Dong N . The inhibitory effect of levo-tetrahydropalmatine on the methamphetamine-induced spatial memory impairment in mice. Neurosci Lett. 2018; 672:34-39. DOI: 10.1016/j.neulet.2018.02.018. View

17.

Vinaixa M, Rodriguez M, Rull A, Beltran R, Blade C, Brezmes J . Metabolomic assessment of the effect of dietary cholesterol in the progressive development of fatty liver disease. J Proteome Res. 2010; 9(5):2527-38. DOI: 10.1021/pr901203w. View

18.

Qu Z, Zhang J, Yang H, Huo L, Gao J, Chen H . Protective effect of tetrahydropalmatine against d-galactose induced memory impairment in rat. Physiol Behav. 2015; 154:114-25. DOI: 10.1016/j.physbeh.2015.11.016. View

19.

Pal R, Tiwari P, Nath R, Pant K . Role of neuroinflammation and latent transcription factors in pathogenesis of Parkinson's disease. Neurol Res. 2016; 38(12):1111-1122. DOI: 10.1080/01616412.2016.1249997. View

20.

Alkhouri N, Dixon L, Feldstein A . Lipotoxicity in nonalcoholic fatty liver disease: not all lipids are created equal. Expert Rev Gastroenterol Hepatol. 2009; 3(4):445-51. PMC: 2775708. DOI: 10.1586/egh.09.32. View