Changes in Duodenal CD163-positive Cells in Dogs with Chronic Enteropathy After Successful Treatment

Overview

Journal Innate Immun

Publisher Sage Publications

Specialties Allergy & Immunology
Microbiology

Date 2018 Sep 19

PMID 30223681

Citations 5

Authors

Julien Rs Dandrieux

Lina Maria Martinez Lopez

Andrew Stent

Albert Jergens

Karin Allenspach

Cameron J Nowell

Simon M Firestone

Wayne Kimpton

Caroline S Mansfield

Affiliations

Soon will be listed here.

Abstract

Chronic enteropathy (CE) in dogs is characterized retrospectively per treatment response as food-responsive enteropathy (FRE), antibiotic-responsive enteropathy (ARE), and immunosuppressant-responsive enteropathy (IRE) - the latter most resembling inflammatory bowel disease in people. The aim of this study was to characterize duodenal macrophages (Mϕ) in CE using immunohistochemistry; with calprotectin (CAL) as a marker of early differentiated Mϕ and CD163 expression as a marker for resident Mϕ in the duodenum before and after treatment. Prior to treatment, dogs with FRE and IRE had a lower CD163/CAL ratio than control dogs (CTRL) in crypts; this increased significantly and normalized compared with CTRL after treatment. Conversely, the CD163/CAL ratio in dogs with ARE was comparable to that in healthy dogs before and after treatment. In summary, these results suggest that Mϕ play a role in the pathogenesis of CE in FRE and IRE, with a decrease in resident Mϕ and an increase in early differentiated Mϕ, but not in ARE dogs. Mϕ normalize after successful treatment.

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