A Gene Essential for Agrobacterium Virulence is Homologous to a Family of Positive Regulatory Loci

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Nov 1

PMID 3022288

Citations 129

Authors

S C Winans

P R Ebert

S E Stachel

M P Gordon

E W Nester

Affiliations

Soon will be listed here.

Abstract

The vir region of Agrobacterium tumefaciens spans at least six transcriptional loci required for crown gall tumorigenesis. The transcriptional induction of two of these vir loci in response to cocultivation with tobacco suspension cells was measured by using bacteria containing mutations in each of the six vir loci located on the Ti plasmid. Induction of these vir genes occurred only in bacteria that had functional copies of virA and virG. The nucleic acid sequence of a 1.25-kilobase clone encompassing virG contains one open reading frame capable of coding for a protein of about 30,000 daltons. The amino acid sequence of the predicted virG product is homologous to that of eight bacterial proteins, including that of the ompR gene of Escherichia coli. Most, although not all, of these proteins, like VirG, are positive regulatory elements.

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References

Drummond M, Whitty P, Wootton J . Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. EMBO J. 1986; 5(2):441-7. PMC: 1166750. DOI: 10.1002/j.1460-2075.1986.tb04230.x. View

FERRARI F, Trach K, Lecoq D, Spence J, Ferrari E, Hoch J . Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985; 82(9):2647-51. PMC: 397621. DOI: 10.1073/pnas.82.9.2647. View

Simms S, Keane M, Stock J . Multiple forms of the CheB methylesterase in bacterial chemosensing. J Biol Chem. 1985; 260(18):10161-8. View

Drury L, Buxton R . DNA sequence analysis of the dye gene of Escherichia coli reveals amino acid homology between the dye and OmpR proteins. J Biol Chem. 1985; 260(7):4236-42. View

Hernandez-Chico C, Herrero M, Rejas M, San Millan J, Moreno F . Gene ompR and regulation of microcin 17 and colicin e2 syntheses. J Bacteriol. 1982; 152(2):897-900. PMC: 221546. DOI: 10.1128/jb.152.2.897-900.1982. View

Nixon B, Ronson C, Ausubel F . Two-component regulatory systems responsive to environmental stimuli share strongly conserved domains with the nitrogen assimilation regulatory genes ntrB and ntrC. Proc Natl Acad Sci U S A. 1986; 83(20):7850-4. PMC: 386820. DOI: 10.1073/pnas.83.20.7850. View

Mutoh N, Simon M . Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli. J Bacteriol. 1986; 165(1):161-6. PMC: 214384. DOI: 10.1128/jb.165.1.161-166.1986. View

MacFarlane S, Merrick M . The nucleotide sequence of the nitrogen regulation gene ntrB and the glnA-ntrBC intergenic region of Klebsiella pneumoniae. Nucleic Acids Res. 1985; 13(21):7591-606. PMC: 322073. DOI: 10.1093/nar/13.21.7591. View

Krikos A, Mutoh N, Boyd A, Simon M . Sensory transducers of E. coli are composed of discrete structural and functional domains. Cell. 1983; 33(2):615-22. DOI: 10.1016/0092-8674(83)90442-7. View

10.

Das A, Stachel S, Ebert P, Allenza P, Montoya A, Nester E . Promoters of Agrobacterium tumefaciens Ti-plasmid virulence genes. Nucleic Acids Res. 1986; 14(3):1355-64. PMC: 339509. DOI: 10.1093/nar/14.3.1355. View

11.

Stachel S, Nester E . The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens. EMBO J. 1986; 5(7):1445-54. PMC: 1166964. DOI: 10.1002/j.1460-2075.1986.tb04381.x. View

12.

Albin R, Weber R, Silverman P . The Cpx proteins of Escherichia coli K12. Immunologic detection of the chromosomal cpxA gene product. J Biol Chem. 1986; 261(10):4698-705. View

13.

Comeau D, Ikenaka K, Tsung K, Inouye M . Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol. 1985; 164(2):578-84. PMC: 214291. DOI: 10.1128/jb.164.2.578-584.1985. View

14.

Hall M, Silhavy T . Genetic analysis of the major outer membrane proteins of Escherichia coli. Annu Rev Genet. 1981; 15:91-142. DOI: 10.1146/annurev.ge.15.120181.000515. View

15.

Shinagawa H, Makino K, Nakata A . Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB. J Mol Biol. 1983; 168(3):477-88. DOI: 10.1016/s0022-2836(83)80297-6. View

16.

Dixon R . The genetic complexity of nitrogen fixation. The ninth Fleming lecture. J Gen Microbiol. 1984; 130(11):2745-55. DOI: 10.1099/00221287-130-11-2745. View

17.

Stachel S, An G, Flores C, Nester E . A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 1985; 4(4):891-8. PMC: 554276. DOI: 10.1002/j.1460-2075.1985.tb03715.x. View

18.

Lipman D, Pearson W . Rapid and sensitive protein similarity searches. Science. 1985; 227(4693):1435-41. DOI: 10.1126/science.2983426. View

19.

Garfinkel D, Nester E . Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol. 1980; 144(2):732-43. PMC: 294723. DOI: 10.1128/jb.144.2.732-743.1980. View

20.

Stock A, Koshland Jr D, Stock J . Homologies between the Salmonella typhimurium CheY protein and proteins involved in the regulation of chemotaxis, membrane protein synthesis, and sporulation. Proc Natl Acad Sci U S A. 1985; 82(23):7989-93. PMC: 391427. DOI: 10.1073/pnas.82.23.7989. View