MicroRNA-552 Promotes Hepatocellular Carcinoma Progression by Downregulating WIF1

Overview

Journal Int J Mol Med

Specialty Genetics

Date 2018 Sep 18

PMID 30221686

Citations 9

Authors

Chao Li

Zi Wang

Shuangjiang Chen

Jingyao Zhang

Kai Qu

Chang Liu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs/miRs) are involved in the metastasis of hepatocellular carcinoma (HCC). In the present study, it was demonstrated that miR‑552 was upregulated in HCC tissues. High miR‑552 expression was associated with malignant clinicopathological features and decreased survival rates. The in vitro results indicated that miR‑552 overexpression promoted migration, invasion and epithelial‑mesenchymal transition in Hep3B cells. However, the knockdown of miR‑552 inhibited its oncogenic roles in Huh‑7 cells. Additionally, Wnt inhibitory factor 1 (WIF1) was demonstrated to be a direct target of miR‑552 in Hep3B and Huh‑7 cells. Additional experiments identified that miR‑552 promotes β‑catenin expression by increasing the phosphorylation of GSK3β at Ser9. In conclusion, the results suggested that miR‑552 may promote HCC progression by blocking WIF1‑mediated GSK3β dephosphorylation. miR‑552 may be a biomarker for predicting the outcomes of patients with HCC.

Citing Articles

-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits.

Chen G, Pu G, Wang L, Li Y, Liu T, Li H Front Immunol. 2023; 14:1201455.

PMID: 37559722 PMC: 10408446. DOI: 10.3389/fimmu.2023.1201455.

Role of non-coding RNAs and exosomal non-coding RNAs in retinoblastoma progression.

Ahangar Davoodi N, Najafi S, Ghale-Noie Z, Piranviseh A, Mollazadeh S, Ahmadi Asouri S Front Cell Dev Biol. 2023; 10:1065837.

PMID: 36619866 PMC: 9816416. DOI: 10.3389/fcell.2022.1065837.

[Overexpression of miR-607 inhibits hepatocellular carcinoma cell growth and metastasis by down-regulating TRPC5].

Li C, Chen S, Jiang Y Nan Fang Yi Ke Da Xue Xue Bao. 2022; 42(11):1587-1593.

PMID: 36504050 PMC: 9742782. DOI: 10.12122/j.issn.1673-4254.2022.11.01.

miR-340 Promotes Retinoblastoma Cell Proliferation, Migration and Invasion Through Targeting WIF1.

Li K, Han F, Wu Y, Wang X Onco Targets Ther. 2021; 14:3635-3648.

PMID: 34113129 PMC: 8187089. DOI: 10.2147/OTT.S302800.

MicroRNA-552 expression in colorectal cancer and its clinicopathological significance.

Im J, Nam S, Lee H J Pathol Transl Med. 2021; 55(2):125-131.

PMID: 33596633 PMC: 7987523. DOI: 10.4132/jptm.2021.01.17.

References

Liu H, Lei C, He Q, Pan Z, Xiao D, Tao Y . Nuclear functions of mammalian MicroRNAs in gene regulation, immunity and cancer. Mol Cancer. 2018; 17(1):64. PMC: 5822656. DOI: 10.1186/s12943-018-0765-5. View

Gunes Yegin E, Oymaci E, Karatay E, Coker A . Progress in surgical and nonsurgical approaches for hepatocellular carcinoma treatment. Hepatobiliary Pancreat Dis Int. 2016; 15(3):234-56. DOI: 10.1016/s1499-3872(16)60097-8. View

Chen J, Rajasekaran M, Hui K . Atypical regulators of Wnt/β-catenin signaling as potential therapeutic targets in Hepatocellular Carcinoma. Exp Biol Med (Maywood). 2017; 242(11):1142-1149. PMC: 5478001. DOI: 10.1177/1535370217705865. View

Lou W, Liu J, Gao Y, Zhong G, Chen D, Shen J . MicroRNAs in cancer metastasis and angiogenesis. Oncotarget. 2018; 8(70):115787-115802. PMC: 5777813. DOI: 10.18632/oncotarget.23115. View

Brabletz T, Kalluri R, Nieto M, Weinberg R . EMT in cancer. Nat Rev Cancer. 2018; 18(2):128-134. DOI: 10.1038/nrc.2017.118. View

Schmid S, Sathe A, Guerth F, Seitz A, Heck M, Maurer T . Wntless promotes bladder cancer growth and acts synergistically as a molecular target in combination with cisplatin. Urol Oncol. 2017; 35(9):544.e1-544.e10. DOI: 10.1016/j.urolonc.2017.04.015. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Duseja A . Staging of hepatocellular carcinoma. J Clin Exp Hepatol. 2015; 4(Suppl 3):S74-9. PMC: 4284240. DOI: 10.1016/j.jceh.2014.03.045. View

Cui Y, Han X, Lin Y, Lv W, Wang Y . TNF-α was involved in calcium hydroxide-promoted osteogenic differentiation of human DPSCs through NF-κB/p38MAPK/Wnt pathway. Pharmazie. 2018; 72(6):329-333. DOI: 10.1691/ph.2017.7450. View

10.

Wu C, Zhuang Y, Jiang S, Liu S, Zhou J, Wu J . Interaction between Wnt/β-catenin pathway and microRNAs regulates epithelial-mesenchymal transition in gastric cancer (Review). Int J Oncol. 2016; 48(6):2236-46. DOI: 10.3892/ijo.2016.3480. View

11.

Zhang Y, Wei Y, Li X, Liang X, Wang L, Song J . microRNA-874 suppresses tumor proliferation and metastasis in hepatocellular carcinoma by targeting the DOR/EGFR/ERK pathway. Cell Death Dis. 2018; 9(2):130. PMC: 5833540. DOI: 10.1038/s41419-017-0131-3. View

12.

Guo H, Zhou S, Tan L, Wu X, Wu Z, Ran R . Clinicopathological significance of WIF1 hypermethylation in NSCLC, a meta-analysis and literature review. Oncotarget. 2016; 8(2):2550-2557. PMC: 5356219. DOI: 10.18632/oncotarget.13707. View

13.

Anton L, DeVine A, Sierra L, Brown A, Elovitz M . miR-143 and miR-145 disrupt the cervical epithelial barrier through dysregulation of cell adhesion, apoptosis and proliferation. Sci Rep. 2017; 7(1):3020. PMC: 5465080. DOI: 10.1038/s41598-017-03217-7. View

14.

Fang L, Wang X, Sun B, Zhang X, Zhu X, Yu Z . Expression, regulation and mechanism of action of the miR-17-92 cluster in tumor cells (Review). Int J Mol Med. 2017; 40(6):1624-1630. PMC: 5716450. DOI: 10.3892/ijmm.2017.3164. View

15.

Li C, Jiang Y, Miao R, Qu K, Zhang J, Liu C . MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the PTP4A1/c-Src axis. Int J Oncol. 2018; 52(2):536-546. DOI: 10.3892/ijo.2017.4224. View

16.

Wang X, Wu M, Liu F, Li Y, Li N, Li G . Differential miRNA expression and their target genes between NGX6-positive and negative colon cancer cells. Mol Cell Biochem. 2010; 345(1-2):283-90. DOI: 10.1007/s11010-010-0582-7. View

17.

Tang A, Hallouch O, Chernyak V, Kamaya A, Sirlin C . Epidemiology of hepatocellular carcinoma: target population for surveillance and diagnosis. Abdom Radiol (NY). 2017; 43(1):13-25. DOI: 10.1007/s00261-017-1209-1. View

18.

Rankin S, McCracken K, Luedeke D, Han L, Wells J, Shannon J . Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis. Dev Biol. 2017; 434(1):121-132. PMC: 5785443. DOI: 10.1016/j.ydbio.2017.11.018. View

19.

Tian Q, Xiao Y, Wu Y, Liu Y, Song Z, Gao W . MicroRNA-33b suppresses the proliferation and metastasis of hepatocellular carcinoma cells through the inhibition of Sal-like protein 4 expression. Int J Mol Med. 2016; 38(5):1587-1595. DOI: 10.3892/ijmm.2016.2754. View

20.

Wu W, Wang W, Yao W, Li G . Suppressive effects of microRNA-16 on the proliferation, invasion and metastasis of hepatocellular carcinoma cells. Int J Mol Med. 2015; 36(6):1713-9. DOI: 10.3892/ijmm.2015.2379. View