Expression of the Complete Human T-cell Leukemia Virus Type I PX Coding Sequence As a Functional Protein in Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Oct 1

PMID 3020538

Citations 19

Authors

C Z Giam

M Nerenberg

G Khoury

G Jay

Affiliations

Soon will be listed here.

Abstract

Human T-cell leukemia virus type I (HTLV-I), a virus associated with adult T-cell leukemia, contains a long open reading frame (LOR) in the 3' end of its genome between the env region and the 3' long terminal repeat (LTR). This open reading frame encodes a 40-kDa protein (designated p40x) that has been implicated as a positive control element for transcription from the HTLV-I LTR in a phenomenon known as trans-activation. We now report the expression of the complete p40x coding sequence as a 40-kDa protein in Escherichia coli. The p40x protein produced in bacteria is shown, using the protoplast fusion technique, to possess biological activity by its ability to trans-activate a HTLV-I LTR-chloramphenicol acetyltransferase plasmid that is stably integrated into the genome of mouse L cells. This stimulatory activity could be detected within 2 hr after fusion, suggesting the possibility of a direct role for p40x in trans-activation of the HTLV-I LTR. The production of p40x in large quantities in E. coli, together with the rapid protoplast fusion assay for its biological activity, should facilitate the analysis of p40x mutants and the elucidation of the molecular mechanism of trans-activation.

Citing Articles

Induction of tumor necrosis factor alpha in human neuronal cells by extracellular human T-cell lymphotropic virus type 1 Tax.

Cowan E, Alexander R, Daniel S, Kashanchi F, Brady J J Virol. 1997; 71(9):6982-9.

PMID: 9261427 PMC: 191983. DOI: 10.1128/JVI.71.9.6982-6989.1997.

Distinct regions in human T-cell lymphotropic virus type I tax mediate interactions with activator protein CREB and basal transcription factors.

Adya N, Giam C J Virol. 1995; 69(3):1834-41.

PMID: 7853524 PMC: 188794. DOI: 10.1128/JVI.69.3.1834-1841.1995.

Transactivation of the human T-cell lymphotropic virus type 1 Tax1-responsive 21-base-pair repeats requires Holo-TFIID and TFIIA.

Duvall J, Kashanchi F, Cvekl A, Radonovich M, Piras G, Brady J J Virol. 1995; 69(8):5077-86.

PMID: 7609077 PMC: 189325. DOI: 10.1128/JVI.69.8.5077-5086.1995.

Characterization of cellular factors that interact with the human T-cell leukemia virus type I p40x-responsive 21-base-pair sequence.

Jeang K, Boros I, Brady J, Radonovich M, Khoury G J Virol. 1988; 62(12):4499-509.

PMID: 3263510 PMC: 253560. DOI: 10.1128/JVI.62.12.4499-4509.1988.

Identification of p40x-responsive regulatory sequences within the human T-cell leukemia virus type I long terminal repeat.

Brady J, Jeang K, Duvall J, Khoury G J Virol. 1987; 61(7):2175-81.

PMID: 3035218 PMC: 254240. DOI: 10.1128/JVI.61.7.2175-2181.1987.

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