MicroRNA Dysregulation and Steroid Hormone Receptor Expression in Uterine Tissues of Rats with Endometriosis During the Implantation Window

Overview

Journal Chin Med J (Engl)

Specialty General Medicine

Date 2018 Sep 12

PMID 30203794

Citations 16

Authors

Han Cai

Xin-Xin Zhu

Zhan-Fei Li

Ya-Pei Zhu

Jing-He Lang

Affiliations

Soon will be listed here.

Abstract

Background: Estrogen receptor (ER) and progesterone receptor (PR) are involved in endometriosis, but the involvement of microRNAs (miRNAs) is unknown. The aim of the study was to explore the correlation between miRNA and ER/PR in uterine tissues of rats with endometriosis during the implantation window.

Methods: Twenty female Sprague-Dawley rats were randomized in three groups: endometriosis (n = 7), fat tissue control (n = 6), and normal (n = 7) groups. The female rats were mated and sacrificed on day 5 (implantation). Uterine tissues were obtained for hematoxylin-eosin staining, immunohistochemistry, and miRNA expression. Reverse transcription polymerase chain reaction (RT-PCR) was used to validate the expression of rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p.

Results: The 475 miRNAs were found to differentially express between the endometriosis and normal control groups, with 127 being upregulated and 348 being downregulated. Expression of five miRNAs (rno-miR-29c-3p, rno-miR-34c-5p, rno-miR-141-5p, rno-miR-24-1-5p, and rno-miR-490-5p) were validated by RT-PCR and found to be differentially expressed among the three groups. Expression of ER and PR proteins (immunohistochemistry) in the glandular epithelium and endometrial stroma was significantly different among the three groups (all P < 0.05). Five miRNAs were involved in pathways probably taking part in implantation and fertility.

Conclusions: The results suggested that miRNAs, ER, and PR could play important roles in the embryo implantation period of rats with endometriosis. These miRNAs might play a role in endometrial receptivity in endometriosis.

Citing Articles

Decreased expression of and in endometriosis during the secretory phase of menstrual cycle: Insights from a case-control study on molecular biomarkers and disease-related infertility.

Hagh Y, Ahmadifard M, Esmaelzadeh S, Abbaszadeh S, Shokrzadeh N Int J Reprod Biomed. 2025; 22(12):1003-1014.

PMID: 39968362 PMC: 11830922. DOI: 10.18502/ijrm.v22i12.18066.

Exploring the Role of MicroRNAs in Progesterone and Estrogen Receptor Expression in Endometriosis.

Hon J, Abdul Wahab N, Abdul Karim A, Mokhtar N, Mokhtar M Biomedicines. 2024; 12(10).

PMID: 39457531 PMC: 11504708. DOI: 10.3390/biomedicines12102218.

Expression and clinical significance of miR-141-5p as a biomarker in the serum of patients with early spontaneous abortion.

Che X, Wang X, Wang L, Xu L, Zou L, Ma T Clinics (Sao Paulo). 2024; 79:100327.

PMID: 38330788 PMC: 10864754. DOI: 10.1016/j.clinsp.2024.100327.

lncRNA TTTY14 participates in the progression of repeated implantation failure by regulating the miR-6088/SEMA5A axis.

Yu L, Ye J, Chen Q, Hong Q J Assist Reprod Genet. 2024; 41(3):727-737.

PMID: 38294620 PMC: 10957803. DOI: 10.1007/s10815-024-03032-w.

Identification of Potentially Novel Molecular Targets of Endometrial Cancer Using a Non-Biased Proteomic Approach.

Taylor A, Konje J, Ayakannu T Cancers (Basel). 2023; 15(18).

PMID: 37760635 PMC: 10527058. DOI: 10.3390/cancers15184665.

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