Biomimetic Oyster Shell-replicated Topography Alters the Behaviour of Human Skeletal Stem Cells

Overview

Journal J Tissue Eng

Date 2018 Sep 12

PMID 30202512

Citations 7

Authors

Shona J Waddell

Maria C de Andres

Penelope M Tsimbouri

Enateri V Alakpa

Maggie Cusack

Matthew J Dalby

Richard Oc Oreffo

Affiliations

Soon will be listed here.

Abstract

The regenerative potential of skeletal stem cells provides an attractive prospect to generate bone tissue needed for musculoskeletal reparation. A central issue remains efficacious, controlled cell differentiation strategies to aid progression of cell therapies to the clinic. The nacre surface from shells is known to enhance bone formation. However, to date, there is a paucity of information on the role of the topography of surfaces, nacre and prism. To investigate this, nacre and prism topographical features were replicated onto polycaprolactone and skeletal stem cell behaviour on the surfaces studied. Skeletal stem cells on nacre surfaces exhibited an increase in cell area, increase in expression of osteogenic markers ( < 0.05) and ( < 0.01) and increased metabolite intensity ( < 0.05), indicating a role of nacre surface to induce osteogenic differentiation, while on prism surfaces, skeletal stem cells did not show alterations in cell area or osteogenic marker expression and a decrease in metabolite intensity ( < 0.05), demonstrating a distinct role for the prism surface, with the potential to maintain the skeletal stem cell phenotype.

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