A PECTIN METHYLESTERASE Gene at the Maize Ga1 Locus Confers Male Function in Unilateral Cross-incompatibility

Overview

Journal Nat Commun

Specialty Biology

Date 2018 Sep 12

PMID 30202064

Citations 32

Authors

Zhaogui Zhang

Baocai Zhang

Zhibin Chen

Dongmei Zhang

Huairen Zhang

Hang Wang

Yue Zhang

Darun Cai

Juan Liu

Senlin Xiao

Yanqing Huo

Jie Liu

Lanjun Zhang

Mingming Wang

Xu Liu

Yongbiao Xue

Li Zhao

Yihua Zhou

Huabang Chen

Affiliations

Soon will be listed here.

Abstract

Unilateral cross-incompatibility (UCI) is a unidirectional inter/intra-population reproductive barrier when both parents are self-compatible. Maize Gametophyte factor1 (Ga1) is an intraspecific UCI system and has been utilized in breeding. However, the mechanism underlying maize UCI specificity has remained mysterious for decades. Here, we report the cloning of ZmGa1P, a pollen-expressed PECTIN METHYLESTERASE (PME) gene at the Ga1 locus that can confer the male function in the maize UCI system. Homozygous transgenic plants expressing ZmGa1P in a ga1 background can fertilize Ga1-S plants and can be fertilized by pollen of ga1 plants. ZmGa1P protein is predominantly localized to the apex of growing pollen tubes and may interact with another pollen-specific PME protein, ZmPME10-1, to maintain the state of pectin methylesterification required for pollen tube growth in Ga1-S silks. Our study discloses a PME-mediated UCI mechanism and provides a tool to manipulate hybrid breeding.

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