Biophysical Properties of Single Rotavirus Particles Account for the Functions of Protein Shells in a Multilayered Virus

Overview

Journal Elife

Specialty Biology

Date 2018 Sep 12

PMID 30201094

Citations 18

Authors

Manuel Jimenez-Zaragoza

Marina Pl Yubero

Esther Martin-Forero

Jose R Caston

David Reguera

Daniel Luque

Pedro J de Pablo

Javier M Rodriguez

Affiliations

Soon will be listed here.

Abstract

The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion.

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