Effects of Ascorbic Acid on Alkaline Phosphatase Activity and Hormone Responsiveness in the Osteoblastic Osteosarcoma Cell Line UMR-106

Overview

Journal Calcif Tissue Int

Specialty Pathology

Date 1986 Sep 1

PMID 3019492

Citations 10

Authors

T Sugimoto

M Nakada

M Fukase

Y Imai

Y Kinoshita

T Fujita

Affiliations

Soon will be listed here.

Abstract

L-ascorbic acid at physiological concentrations (10 micrograms/ml) increased alkaline phosphatase activity in the osteoblastlike rat osteosarcoma cell line, UMR-106. The increase was dose-dependent and detectable at 6 hours after the addition of 100 micrograms/ml ascorbic acid to the medium. Treatment of the cells with 100 micrograms/ml ascorbic acid potentiated the response of cAMP to both PTH and PGE1, while cell growth was inhibited. Furthermore, the number of colonies formed by the cells grown in the soft agar was significantly reduced by increasing concentrations of ascorbic acid. These results indicate that ascorbic acid might play some role in the differentiation of osteoblasts.

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