Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking

Overview

Journal Sci Rep

Specialty Science

Date 2018 Sep 6

PMID 30181604

Citations 11

Authors

Sang-Soo Han

Hye-Eun Shim

Soon-Jung Park

Byoung-Chul Kim

Dong-Eun Lee

Hyung-Min Chung

Sung-Hwan Moon

Sun-Woong Kang

Affiliations

Soon will be listed here.

Abstract

Metabolic labeling is one of the most powerful methods to label the live cell for in vitro and in vivo tracking. However, the cellular mechanisms by modified glycosylation due to metabolic agents are not fully understood. Therefore, metabolic labeling has not yet been widely used in EPC tracking and labeling. In this study, cell functional properties such as proliferation, migration and permeability and gene expression patterns of metabolic labeling agent-treated hUCB-EPCs were analyzed to demonstrate cellular effects of metabolic labeling agents. As the results, 10 μM Ac4ManNAz treatment had no effects on cellular function or gene regulations, however, higher concentration of Ac4ManNAz (>20 μM) led to the inhibition of functional properties (proliferation rate, viability and rate of endocytosis) and down-regulation of genes related to cell adhesion, PI3K/AKT, FGF and EGFR signaling pathways. Interestingly, the new blood vessel formation and angiogenic potential of hUCB-EPCs were not affected by Ac4ManNAz concentration. Based on our results, we suggest 10 μM as the optimal concentration of Ac4ManNAz for in vivo hUCB-EPC labeling and tracking. Additionally, we expect that our approach can be used for understanding the efficacy and safety of stem cell-based therapy in vivo.

Citing Articles

Glycoengineering with neuraminic acid analogs to label lipooligosaccharides and detect native sialyltransferase activity in gram-negative bacteria.

Alvarado-Melendez E, de Jong H, Hartman J, Ong J, Wosten M, Wennekes T Glycobiology. 2024; 34(10).

PMID: 39244665 PMC: 11413452. DOI: 10.1093/glycob/cwae071.

Clickable Polysaccharides for Biomedical Applications: A Comprehensive Review.

Yazdi M, Sajadi S, Seidi F, Rabiee N, Fatahi Y, Rabiee M Prog Polym Sci. 2023; 133.

PMID: 37779922 PMC: 10540641. DOI: 10.1016/j.progpolymsci.2022.101590.

The Essential Function of miR-5739 in Embryonic Muscle Development.

Lee J, Kim M, Lee J, Lee D, Park C, Lee D Int J Stem Cells. 2023; 16(2):145-155.

PMID: 36823980 PMC: 10226859. DOI: 10.15283/ijsc22187.

Mesenchymal Stem Cell-Mediated Deep Tumor Delivery of Gold Nanorod for Photothermal Therapy.

Yun W, Shim M, Lim S, Song S, Kim J, Yang S Nanomaterials (Basel). 2022; 12(19).

PMID: 36234538 PMC: 9565344. DOI: 10.3390/nano12193410.

From Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering.

Idiago-Lopez J, Moreno-Antolin E, Eceiza M, Aizpurua J, Grazu V, de la Fuente J Bioconjug Chem. 2022; 33(9):1620-1633.

PMID: 35857350 PMC: 9501912. DOI: 10.1021/acs.bioconjchem.2c00230.

References

Chen L, Liang J . Metabolic monosaccharides altered cell responses to anticancer drugs. Eur J Pharm Biopharm. 2012; 81(2):339-45. PMC: 3556389. DOI: 10.1016/j.ejpb.2012.03.012. View

Arbab A, Yocum G, Rad A, Khakoo A, Fellowes V, Read E . Labeling of cells with ferumoxides-protamine sulfate complexes does not inhibit function or differentiation capacity of hematopoietic or mesenchymal stem cells. NMR Biomed. 2005; 18(8):553-9. DOI: 10.1002/nbm.991. View

Li Y, Ren L, Guo G, Cannella L, Chernaya V, Samuel S . Endothelial progenitor cells in ischemic stroke: an exploration from hypothesis to therapy. J Hematol Oncol. 2015; 8:33. PMC: 4446087. DOI: 10.1186/s13045-015-0130-8. View

Hayakawa K, Hirosawa M, Tabei Y, Arai D, Tanaka S, Murakami N . Epigenetic switching by the metabolism-sensing factors in the generation of orexin neurons from mouse embryonic stem cells. J Biol Chem. 2013; 288(24):17099-110. PMC: 3682516. DOI: 10.1074/jbc.M113.455899. View

Dube D, Prescher J, Quang C, Bertozzi C . Probing mucin-type O-linked glycosylation in living animals. Proc Natl Acad Sci U S A. 2006; 103(13):4819-24. PMC: 1405625. DOI: 10.1073/pnas.0506855103. View

Bliss T, Guzman R, Daadi M, Steinberg G . Cell transplantation therapy for stroke. Stroke. 2007; 38(2 Suppl):817-26. DOI: 10.1161/01.STR.0000247888.25985.62. View

Dommerholt J, Schmidt S, Temming R, Hendriks L, Rutjes F, van Hest J . Readily accessible bicyclononynes for bioorthogonal labeling and three-dimensional imaging of living cells. Angew Chem Int Ed Engl. 2010; 49(49):9422-5. PMC: 3021724. DOI: 10.1002/anie.201003761. View

Ellis S, Tanentzapf G . Integrin-mediated adhesion and stem-cell-niche interactions. Cell Tissue Res. 2009; 339(1):121-30. DOI: 10.1007/s00441-009-0828-4. View

Soncin F, Ward C . The function of e-cadherin in stem cell pluripotency and self-renewal. Genes (Basel). 2014; 2(1):229-59. PMC: 3924836. DOI: 10.3390/genes2010229. View

10.

Song E, Lu C, Fang L, Yang W . Culture and identification of endothelial progenitor cells from human umbilical cord blood. Int J Ophthalmol. 2012; 3(1):49-53. PMC: 3340652. DOI: 10.3980/j.issn.2222-3959.2010.01.11. View

11.

Lee S, Yoon H, Na J, Jeon S, Lim S, Koo H . In vivo stem cell tracking with imageable nanoparticles that bind bioorthogonal chemical receptors on the stem cell surface. Biomaterials. 2017; 139:12-29. DOI: 10.1016/j.biomaterials.2017.05.050. View

12.

Laughlin S, Bertozzi C . Metabolic labeling of glycans with azido sugars and subsequent glycan-profiling and visualization via Staudinger ligation. Nat Protoc. 2007; 2(11):2930-44. DOI: 10.1038/nprot.2007.422. View

13.

Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T . Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997; 275(5302):964-7. DOI: 10.1126/science.275.5302.964. View

14.

Higuchi T, Anton M, Saraste A, Dumler K, Pelisek J, Nekolla S . Reporter gene PET for monitoring survival of transplanted endothelial progenitor cells in the rat heart after pretreatment with VEGF and atorvastatin. J Nucl Med. 2009; 50(11):1881-6. DOI: 10.2967/jnumed.109.067801. View

15.

Madl C, Heilshorn S . Bioorthogonal Strategies for Engineering Extracellular Matrices. Adv Funct Mater. 2019; 28(11). PMC: 6761700. DOI: 10.1002/adfm.201706046. View

16.

Comer F, Hart G . O-Glycosylation of nuclear and cytosolic proteins. Dynamic interplay between O-GlcNAc and O-phosphate. J Biol Chem. 2000; 275(38):29179-82. DOI: 10.1074/jbc.R000010200. View

17.

Akiyama S . Integrins in cell adhesion and signaling. Hum Cell. 1996; 9(3):181-6. View

18.

Rowland T, Miller L, Blaschke A, Doss E, Bonham A, Hikita S . Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 2009; 19(8):1231-40. DOI: 10.1089/scd.2009.0328. View

19.

Mitchell K, Weiss M, Mitchell B, Martin P, Davis D, Morales L . Matrix cells from Wharton's jelly form neurons and glia. Stem Cells. 2003; 21(1):50-60. DOI: 10.1634/stemcells.21-1-50. View

20.

Finney M, Greco N, Haynesworth S, Martin J, Hedrick D, Swan J . Direct comparison of umbilical cord blood versus bone marrow-derived endothelial precursor cells in mediating neovascularization in response to vascular ischemia. Biol Blood Marrow Transplant. 2006; 12(5):585-93. DOI: 10.1016/j.bbmt.2005.12.037. View